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Rabbit Monoclonal Antibody preparation by phage displayIntroduction
Leading Biology dedicated itself from the first day to develop monoclonal antibodies almost exclusively. We have an onsite vivarium and working to establish a GMP facility soon. We keep a veterinarian on staff to make sure our mice and other animals are in the best health.
We know how to generate an antibody that is specific to the immunizing antigen and that works for the intended application.
Our technical staff with decades of experience in immunology, biochemistry, and molecular biology will be there to guide you through the 10 to 14-week process.
Service Package
|
Service Items |
Time(weeks) |
Peptide |
Molecular |
Phosphate/Methyl/ acetyl-specific |
|||
Peptide design & synthesis |
Peptide design |
~2 |
|
|
|
|
|
|
Peptide synthesis |
√ |
|
|
|
√ |
|
||
conjugation |
√ |
|
√ |
|
√ |
|
||
Immunization |
Immunized 2 rabbits |
~8 |
√ |
√ |
√ |
√ |
√ |
√ |
Elisa detection |
√ |
√ |
√ |
√ |
√ |
√ |
||
cDNA preparation |
prepare cDNA library by RT-PCR |
~1 |
√ |
√ |
√ |
√ |
√ |
√ |
cDNA library QC check |
√ |
√ |
√ |
√ |
√ |
√ |
||
Fab library construction |
obtained VH and VL genes |
10~15 |
√ |
√ |
√ |
√ |
√ |
√ |
primary antibody library construction |
√ |
√ |
√ |
√ |
√ |
√ |
||
Fab library construction |
√ |
√ |
√ |
√ |
√ |
√ |
||
Phage Screening |
phage particles package |
10~15 |
√ |
√ |
√ |
√ |
√ |
√ |
magnetic beads affinity screening |
√ |
√ |
√ |
√ |
√ |
√ |
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Recover positive clones and DNA sequencing |
√ |
√ |
√ |
√ |
√ |
√ |
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Antibody expression |
synthetic of target phage gene and Fc gene |
~5 |
√ |
√ |
√ |
√ |
√ |
√ |
Eukaryotic system expression plasmids construction |
√ |
√ |
√ |
√ |
√ |
√ |
||
Eukaryotic system expression |
√ |
√ |
√ |
√ |
√ |
√ |
||
Stably transfected cell line construction |
|
|
|
|
|
|
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Purification and detection |
Tag affinity purification |
~1 |
√ |
√ |
√ |
√ |
√ |
√ |
Antigen specific affinity purification |
√ |
√ |
√ |
√ |
√ |
√ |
||
Total |
|
7~9 |
|
Service details
Antigen Forms
Polyclonal antibodies can be generated against both soluble and non-soluble recombinant proteins, fusion proteins, gel strips, existing peptide, etc. Live or toxic substances cannot be accepted.
Antigen Quantity
We recommend sending 2-3 mg of the antigen for a rabbit or chicken project and 5-7 mg for a goat project. Extensions or immunochemistry services may require additional antigen.
Antigen Concentration
Leading Biology can work with most protein concentrations as long as this information is provided. 1 mg/ml is a good target range, but higher concentrations are welcome. We consider 0.25 to .5 mg/ml a minimum concentration in most cases to ensure maximum protein volumes in each immunization.
Considerations for Gel Bands
Immunizing with gel bands works well for generating polyclonal antibodies against a purified protein. If providing a gel strip, please stain with coomassie blue, de-stain, rinse and cut out the strip containing the target protein. Please avoid freezing or homogenizing the gel strip so that we can aliquot appropriate quantities of the protein for each immunization. With gel bands, in particular, it is important to maintain a higher concentration of the protein as outlined above.
Shipping Antigens to Leading Biology
Please ship the antigen in a Styrofoam box with ice packs (dry ice isn’t necessary) via FedEx, UPS or DHL Overnight to the address listed on our Order Form.
Related Services
Polyclonal Antibody Serum Preparation
Why Leading Biology?
With over 5 years of experience in custom antibodies, we offer numerous benefits for developing your custom antibody:
• Our industry leading titer guarantees of 1:50,000 eliminate the risk of not obtaining antibodies against peptide antigens.
• Our economies of scale allow us to pass cost savings to you and help maximize your budget.
• 100% of services are transparent throughout.
• Antibodies from our facility have been cited in many published research papers.
• By outsourcing antibody production and characterization needs to us, you can spend more time focused on your research.
• Our ideal location in California ensures that animals benefit from outdoor facilities and mild year-round temperatures.
• Confidentiality is a top priority for all projects. Antibodies that we manufacture belong to you and will not be commercialized.
Contact Information
Please obtain a quote before ordering, and refer to the quote number when you place an order.
Orders are typically confirmed within 12 hours.
Have a Question? Email us info@leadingbiology.com
Order Products: Order Related Products
By Phone: 1-661-524(LBI)-0262 (USA)
No | Headline | Click | Author | Date |
1 | Antibody Production | 2559 | Leading Biology | 2020-01-16 |
2 | Mouse Monoclonal Antibody preparation by phage display | 1327 | Leading Biology | 2019-11-20 |
3 | Rabbit Monoclonal Antibody preparation by phage display | 1717 | Leading Biology | 2019-11-20 |
4 | Antibody Labeling Services | 5765 | Leading Biology | 2019-11-05 |
5 | Custom Monospecific Antibody Production | 1998 | Leading Biology | 2019-11-04 |
6 | Custom Peptide Antibody Production | 6631 | Leading Biology | 2019-11-04 |