Introduction

Compared with common mammalian IgG, chicken IgY antibody has a strong resistance to heat, acid, ionic and have certain enzyme resistance, it can be used as an ideal diagnostic reagent and a drug for prevention and treatment. Leading Biology using SPF level chicken, ensures the pure background and higher production of the antibody, by using international advanced nano latex Immuno amplification technology, acquire antigen with stronger immunogenicity, improve the immunization success possibility, and get chicken antibody with higher titer.

Service Package 

(at least 5 chickens)

Notes

1. Because the laboratory animals are  relatively fragile, and the preparation process is complex, to prevent experimental accidents, the number of chickens for polyclonal antibody preparation project is at least five, each antigen at least immune 2 chickens;

2. We use SPF level animals in the project, and Infectious antigen is strictly prohibited, the customers should acknowledge us if there are specific problems of the antigen, if the customers hiding their antigen information deliberately, and cause significant financial losses of the company, we will reserve the right to the legal responsibility;

3. For chicken polyclonal antibody services, if the customer want antigen specific affinity purification rather than normal specific affinity purification, the price increases 200$;

4. The prices listed above are for reference only, the exact price needs to be decided according to the actual situation of the project.

Classic case 

(Chicken Polyclonal Antibody Service)

Customer Provide: Provide antigen information and  antibody requirements

Process

1. Protein design and synthesis (3-4 weeks)

2. Feeding and immunization of 5 chickens/antigen (4 immunizations, 8 weeks)

3. Blood collection

4. ELISA rescreening (1 week)

5. Purification and delivery (1 week)

Products  

(each antigen, 5 chickens)

1. 0.5-1mL negative serum

2. 10-15mL purified positive serum, ELISA titer was above 1:60,000

3. Complete lab report, remaining antigens

Preparation time: 2-3 months

Successful Sercvice

Publication: Plos One

IF:3.05

Title: Phosphodiesterase Type 4 Inhibitor Rolipram Improves Survival of Spiral Ganglion Neurons In Vitro

Name: Anti-200 kD Neurofilament antibody  

Species: mouse

Type: Chicken polyclonal antibody 

F1. Projections of collapsed confocal scans showed double-stainings with pERK (A) or pCREB (D) antibodies and antibodies directed against neuron-specific 200 kDa neurofilament (B, E) in spiral ganglion cell cultures pre-treated with BDNF (47 h, 30 min) and exposed to Rolipram for 30 min.

Recommended concentration is 1:100,000, using our standard fixation and staining procedure.

Related Services

Rapid Antibody Service

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Rat Polyclonal Antibody Service

Mouse Polyclonal Antibody Service

Rabbit Polyclonal Antibody Service

Polyclonal Antibody Serum Preparation

With over 5 years of experience in custom antibodies, we offer numerous benefits for developing your custom antibody:
• Our industry leading titer guarantees of 1:50,000 eliminate the risk of not obtaining antibodies against peptide antigens.

• Our economies of scale allow us to pass cost savings to you and help maximize your budget.
• 100% of services are transparent throughout.
• Antibodies from our facility have been cited in many published research papers.
• By outsourcing antibody production and characterization needs to us, you can spend more time focused on your research.

• Our ideal location in California ensures that animals benefit from outdoor facilities and mild year-round temperatures.

• Confidentiality is a top priority for all projects. Antibodies that we manufacture belong to you and will not be commercialized.

Please obtain a quote before ordering, and refer to the quote number when you place an order.
Orders are typically confirmed within 12 hours.
Have a Question? Email us info@leadingbiology.com
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By Phone: 1-661-524(LBI)-0262 (USA)