Introduction

Polyclonal antibodies (pAbs) are antibodies that are secreted by different B cell lineages within the body (whereas monoclonal antibodies come from a single cell lineage). They are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.

Service Package

Recommend package

Note:

1. The prices listed above are for reference only, the exact price need to be decided according to actual situation of the project;

2. We will charge 50% of the total price before the project start, and before we send out the final product, we will deliver some samples to customers for test, after customer chose the optimal serum, we charge the rest 50% total price; if the customer gave up asking for the rest samples, the project will be considered filed, and we won’t ask for the rest payment;

3. The prices listed above are for 1 sample only, the price will change if the number of the purified sample increased.

Classic Case 

(Rabbit Polyclonal Antibody Service)

Customer Provide  

Protein

Process

1. Antigen Detection (Less than 1 week)

2. Feeding and immunization of two rabbits/antigen (4 immunizations, 8 weeks)

3. Blood collection

4. Elisa test (1 week)

5. Purification, detection and delivery (1-2 weeks)

Products

(each antigen, 2 rabbits)

1. 3-5mL negative serum

2. 15-20mL positive serum, ELISA titer was above 1:60,000

3. Around 10mL purified positive serum

4. Complete lab report, remaining antigens

Preparation time

About 2 months

Successful case

Publication: Nature (letters)

IF:41.456

Title: A flagellin-induced complex of the receptor FLS2 and BAK1 initiates plant defence 

Name: anti-FLS2 antibody

Species: Arabidopsis thaliana

Type: Rabbit polyclonal antibody

Service details

Antigen Forms

Polyclonal antibodies can be generated against both soluble and non-soluble recombinant proteins, fusion proteins, gel strips, existing peptide, etc. Live or toxic substances cannot be accepted.

Antigen Quantity

We recommend sending 2-3 mg of the antigen for a rabbit or chicken project and 5-7 mg for a goat project. Extensions or immunochemistry services may require additional antigen.

Antigen Concentration

Leading Biology can work with most protein concentrations as long as this information is provided. 1 mg/ml is a good target range, but higher concentrations are welcome. We consider 0.25 to .5 mg/ml a minimum concentration in most cases to ensure maximum protein volumes in each immunization.

Considerations for Gel Bands

Immunizing with gel bands works well for generating polyclonal antibodies against a purified protein. If providing a gel strip, please stain with coomassie blue, de-stain, rinse and cut out the strip containing the target protein. Please avoid freezing or homogenizing the gel strip so that we can aliquot appropriate quantities of the protein for each immunization. With gel bands in particular, it is important to maintain a higher concentration of the protein as outlined above.

Shipping Antigens to Leading Biology

Please ship the antigen in a Styrofoam box with ice packs (dry ice isn’t necessary) via FedEx, UPS or DHL Overnight to the address listed on our Order Form. 

Related Services

Rapid Antibody Service

Chicken Polyclonal Antibody Service

Rat Polyclonal Antibody Service

Mouse Polyclonal Antibody Service

Rabbit Polyclonal Antibody Service

Polyclonal Antibody Serum Preparation

Why Leading Biology?

With over 5 years of experience in custom antibodies, we offer numerous benefits for developing your custom antibody:

• Our industry leading titer guarantees of 1:50,000 eliminate the risk of not obtaining antibodies against peptide antigens.

• Our economies of scale allow us to pass cost savings to you and help maximize your budget.

• 100% of services are transparent throughout.

• Antibodies from our facility have been cited in many published research papers.

• By outsourcing antibody production and characterization needs to us, you can spend more time focused on your research.

• Our ideal location in California ensures that animals benefit from outdoor facilities and mild year-round temperatures.

• Confidentiality is a top priority for all projects. Antibodies that we manufacture belong to you and will not be commercialized.

Contact Information

Please obtain a quote before ordering, and refer to the quote number when you place an order.

Orders are typically confirmed within 12 hours.

Have a Question? Email us info@leadingbiology.com

Order Products: Order Related Products

By Phone: 1-661-524(LBI)-0262 (USA)