Introduction 

The services generate custom rat polyclonal antibodies using peptide antigens designed and optimized using our Antigen Design Tool. Both rat antibody packages come with a guaranteed ELISA titer.

Service Package 

(at least 5 rats)

Note:

1. Because the laboratory animals are  relatively fragile, and the preparation process is complex, to prevent experimental accidents, the number of rats for polyclonal antibody preparation project is at least five, each antigen at least immune 2 rats;

2. We use SPF level rats in the project, and Infectious antigen is strictly prohibited, the customers should acknowledge us if there are specific problems of the antigen, if the customers hiding their antigen information deliberately, and cause significant financial losses of the company, we will reserve the right to the legal responsibility;

3. For rat polyclonal antibody services, if the customer want antigen specific affinity purification rather than normal specific affinity purification, the price increases 200$;

4. The prices listed above are for reference only, the exact price needs to be decided according to the actual situation of the project.

Classic case 

(Rat Polyclonal Antibody Service)

Customer Provide

Provide small molecule information and  antibody requirements

Process

1. Small molecule vector conjugation and testing (1-2 weeks)

2. Feeding and immunization of 5 rats/antigen (4 immunizations, 8 weeks)

3. Blood collection

4. ELISA rescreening (1 week)

5. Purification and delivery (1 week)

Products

(each antigen, 5 rats)

1. 0.5-1mL negative serum

2. 10-15mL purified positive serum, ELISA titer was above 1:60,000

3. Complete lab report, remaining antigens

Preparation time: 2-3 months

Successful Service

Publication: Tumor and Stem Cell Biology

IF:5.013

Title: MicroRNAs Regulate Tumor Angiogenesis Modulated by Endothelial Progenitor Cells

Name: Rat anti-mouse  miR-10b/miR-196b

Species: mouse  

Type: rat monoclonal antibody

F1. Western blot analysis of human endothelial cells showing an increase in levels of HOXD10 protein (40 kDa) after suppression of miR-10b and miR-196b. Upper right, levels of HOXA9 (35 kDa) remained unchanged.

Service Details

Antigen Forms
Polyclonal antibodies can be generated against both soluble and non-soluble recombinant proteins, fusion proteins, gel strips, existing peptide, etc. Live or toxic substances cannot be accepted.

Antigen Quantity
We recommend sending 2-3 mg of the antigen for a rabbit or chicken project and 5-7 mg for a goat project. Extensions or immunochemistry services may require additional antigen.

Antigen Concentration
Leading Biology can work with most protein concentrations as long as this information is provided. 1 mg/ml is a good target range, but higher concentrations are welcome. We consider 0.25 to .5 mg/ml a minimum concentration in most cases to ensure maximum protein volumes in each immunization.

Considerations for Gel Bands
Immunizing with gel bands works well for generating polyclonal antibodies against a purified protein. If providing a gel strip, please stain with coomassie blue, de-stain, rinse and cut out the strip containing the target protein. Please avoid freezing or homogenizing the gel strip so that we can aliquot appropriate quantities of the protein for each immunization. With gel bands in particular, it is important to maintain a higher concentration of the protein as outlined above.

Shipping Antigens to Leading Biology
Please ship the antigen in a Styrofoam box with ice packs (dry ice isn’t necessary) via FedEx, UPS or DHL Overnight to the address listed on our Order Form. 

Related Services

Rapid Antibody Service

Chicken Polyclonal Antibody Service

Rat Polyclonal Antibody Service

Mouse Polyclonal Antibody Service

Rabbit Polyclonal Antibody Service

Polyclonal Antibody Serum Preparation

Why Leading Biology?
With over 5 years of experience in custom antibodies, we offer numerous benefits for developing your custom antibody,

• Our industry leading titer guarantees of 1:50,000 eliminate the risk of not obtaining antibodies against peptide antigens.

• Our economies of scale allow us to pass cost savings to you and help maximize your budget.
• 100% of services are transparent throughout.
• Antibodies from our facility have been cited in many published research papers.
• By outsourcing antibody production and characterization needs to us, you can spend more time focused on your research.

• Our ideal location in California ensures that animals benefit from outdoor facilities and mild year-round temperatures.

• Confidentiality is a top priority for all projects. Antibodies that we manufacture belong to you and will not be commercialized.

Contact Information

Please obtain a quote before ordering, and refer to the quote number when you place an order.
Orders are typically confirmed within 12 hours.
Have a Question? Email us info@leadingbiology.com
Order Products: Order Related Products
By Phone: 1-661-524(LBI)-0262 (USA)