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Location: Home > Custom Services > Antibody Discovery Services > Custom Monospecific Antibody Production

Custom Monospecific Antibody Production

Date: 2019-11-03 Author: Leading Biology Click: 1984

Introduction

Custom Monospecific Antibody Production is our most popular package. It can serve as a cost-effective alternative to monoclonal antibody production for many applications.


By immunizing with a peptide sequence and then affinity purifying the serum against peptide sequence, it is possible to isolate highly specific antibodies that approach the specificity of monoclonal antibodies but provide the superior affinity of polyclonal antibodies.

Service

Details

Rabbits

lProtein Sequence Analysis

lSynthesis of up to a 15aa peptide

lConjugation of 3-5 mg of the peptide to KLH

l13-week Antibody Production Protocol:

u2 Rabbits

uPre-Immune Serum from each animal

u4 Immunizations per animal

u4 Production Bleeds of 20-25 ml of serum per animal

uProvides approximately 160-200 ml of anti-sera

lELISA on 1st Production Bleed (covers both animals)

lAffinity Purification of 25 ml of Serum from 3rd Production Bleed

lReusable Affinity Column

lHandling / Shipment Preparation

Service Process

Service

Details

Peptide

Cost per each additional peptide residue over the 15 residues included with the package

4-Week Extension
(Rabbits)

Provides 1 immunization and 2 additional production bleeds per animal. Provides approximately 80-100 ml of serum. (Covers both animals)

Exsanguination Bleeds
(Rabbits)

Terminal Bleeds that provide approximately 50+ ml of serum per animal. (Covers both animals)

200 ml Affinity Purification

Upgrade of 25 ml Affinity Purification to 200 ml Affinity Purification

Sodium Azide

Preservative for antiserum


Details

Antigen Design:
We provide antigen design assistance at no additional charge. We use our innovative and proprietary combination of algorithms to analyze your protein sequence and predict which regions are immunogenic, unique and exposed.

Conjugation of Peptide to Carrier Protein:
Because peptides are too small to generate an immune response by themselves, conjugation to a carrier protein will be required for immunizations. We recommend coupling peptides to KLH (Keyhole Limpet Hemocyanin).

Terminal Cysteine:
We recommend coupling the peptide to the carrier protein via a cysteine that has been added to the N or C-terminal of the peptide. For this reason, we recommend avoiding sequences that contain internal cysteines whenever possible.

Quantity of Peptide:
We typically synthesize 40-50 mg of immune-grade peptide, with approximately 10 mg being required for conjugation to the carrier protein, ELISA and affinity purification. All unused peptides will be delivered to the customer.

Affinity Column:
The affinity column is included so that additional purifications can be run as needed. Longevity of the columns varies, but the best yields are typically obtained within 6-12 months of the project’s completion.

Titer Guarantees:
We offer one of the highest titer guarantees in the industry, promising at least a 1:50,000 antibody titer against the peptide sequence. For additional details, please read our Guarantee page in our Resources section.

Purified Antibody Yields:
On average, 2-3 mg of peptide-specific antibody will be isolated from affinity purification of 25 ml of antiserum.

Affinity Purification Strategy:
Unless one animal has particularly high antibody titers against the peptide, we recommend affinity-purifying a pool of 25 ml serum taken in a 1:1 ratio from both animals to ensure maximum epitope variability. Comprehensive ELISA testing helps in determining this strategy and in confirming the success of the purification.

Comparison to Monoclonal Antibodies:
Monospecific antibodies offer significant price and time advantages in comparison to monoclonal antibodies. In particular, the affinity purified pool of antibodies against the peptide sequence is highly specific and approaches a monoclonal antibody. At the same time, monospecific antibodies deliver the superior affinity of polyclonal antibodies, which allows for more robust detection of the protein in various assays and conditions. With large scale affinity purifications, large quantities of purified antibody can be obtained.


Why leading biology?
With over 5 years of experience in custom antibodies, we offer numerous benefits for developing your custom antibody:
• Our industry leading titer guarantees of 1:50,000 eliminate the risk of not obtaining antibodies against peptide antigens.

• Our innovative antigen prediction algorithms maximize the probability of targeting peptide sequences that correspond to exposed regions of native proteins.
• Our economies of scale allow us to pass cost savings to you and help maximize your budget.
• 100% of services are transparent throughout.
• Antibodies from our facility have been cited in many published research papers.
• By outsourcing antibody production and characterization needs to us, you can spend more time focused on your research.

• Our ideal location in California ensures that animals benefit from outdoor facilities and mild year-round temperatures.

• Our Monospecific antibody production program generates epitope-specific polyclonal antibodies with superior affinity to monoclonal antibodies and at a fraction of the cost.

• Confidentiality is a top priority for all projects. Antibodies that we manufacture belong to you and will not be commercialized.



Contact Information

Please obtain a quote before ordering, and refer to the quote number when you place an order.
Orders are typically confirmed within 12 hours.
Have a Question? Email us info@leadingbiology.com
Order Products: Order Related Products
By Phone: 1-661-524(LBI)-0262 (USA)


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