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  • Protein Expression and Purification Services
    Leading Biology strives to provide advanced platforms for protein expression and purification service. As a leading biotechnology service supplier, we offer a variety of protein expression and purification systems for high level expression and sample purification. The preparation of high quality, high purity and high-fidelity protein has become a key step in many experiments. Therefore, we deeply understand the importance of building a convenient and easy to use protein high expression and purification system. With our experienced expertise and unique well optimized protocols, we are able to evaluate efficiently and identify the optimal expression condition for a candidate protein to meet our customer’s special requirements. In order to make sure each protein sample the most efficient and closest to the natural host expression system, our company has set up various types of protein expression system. Here we’d like to invite you to review our growing range of expression systems resulting from our experience in cloning, overexpression and purification. Bacterial expression system (Escherichia coli/Bacillus subtilis) Yeast exp...
  • Whole cell expression system based
    The cell-based membrane protein expression system provided by our company, is based on the transfection of recombinant target protein expression plasmids. The diversity expression platform includes all kinds of protein expression sources, including bacteria, yeast, insect cells and mammalian cell lines, Customers only need to provide the information of related target proteins (gene sequence, amino acid sequence, source, protein solubility, etc.). We can start from the first step of downstream protein expression, and perform vector construction, expression, purification, stabilization, even the crystallization of the membrane protein. Under the efforts of our excellent R & D team, we have developed a series of new reagents that could inhibit the degradation, maintain the full-length, natural conformation and the maximum bioactivity of the protein, we also do optimization according to different target proteins. Our customized membrane proteins can meet the requirements of downstream antibody screening, drug discovery, structural determination, function and mechanism research. Category Expression Time ...
  • Protein Expression in Bacteria
    Protein Expression in Bacteria Bacterial expression system is an ideal protein expression system of all time because of its nature. It often acts as the first choice in producing recombinant proteins and antibodies. Many bacteria have already been well characterized and investigated until now. Among the huge number of bacteria, E. coli and B. subtilis cell lines are the most commonly used hosts for recombinant protein expression. They replicate extremely fast under the right condition with a low cost, while the expression level is high, which makes it an efficient and economical way for large scale production. Their genomes have been entirely sequenced and the genetic backgrounds and metabolic pathways are well known. Additionally, because of the simple structures, bacteria are more easily transfected than eukaryotes. These desirable characteristics make the expression procedure easier and quicker. But in some ways, the advantages are also disadvantages. Proteins with few modifications could be expressed in bacteria easily. While there are no nucleus, golgi apparatus and endoplasmic reticulum in bacteria...
  • Unique Protein Production Service
    UPP is an expression cell line which could express foreign protein efficiently. The foreign protein can account for more than 20-30% of the total protein it produced, and its principle is based on an enzyme called Marzf that can hydrolyze host mRNA specifically.This enzyme can specificity recognize the ACA sequence of mRNA, and through codon optimization, remove this sequence from the sequence which encoding exogenous protein, and this could avoid the target protein mRNA having same fate with the host cell protein mRNA. Under the effect of this enzymes, most of the genetic information of the host genes is shielded, while the transcription and translation system still working, this modification can efficiently express the exogenous protein without other interferences. The high Signal-to-noise ratio (SNR) of this technology made the truly high flux protein production came true. With the constant improvement of the system and related technology innovation, by now, we can use this system to achieve the preparation of 90% of proteins, and with the optimization of the production process, we can achieve 40 times concentrated fermentation system for some proteins, and the ...
  • Protein Expression in Cell-Free System
    Service Introduction Cell-free expression system is an external system based on exogenous DNA or mRNA as template, using cell extract which contains cell synthesize organ, protein folding factor and other related enzymes, by adding amino acids and T7 polymerase to achieve the in vitro protein expression system, including the eukaryotic cell-free expression system and the prokaryotic cell-free expression system. Advantages of Cell-Free system 1.Faster data generation: Functional protein can be obtained in only 1 hours, while the cell-based system require several days. 2.Time saving: The expression protein can be used directly in the follow-up experiment, no additional purification needed. 3.It is more suitable for the expression of kinases and other toxic proteins, can be marked with modified tRNA, and add unnatural amino acids at specific sites. 4.One system, multiple applications: Proteins expressed can be used in protein-protein interaction experiment, protein-nucleic acid interaction experiment, and protein modification analysis. Leading Biology cell-Free expression system process 1.Select the appropriate e...
  • Rich in Disulfide Bond Protein Expression
    Service introduction As we all know, the structure of protein which rich in disulfide bond is relatively complex, the solubility of the target protein expressed by the prokaryotic system is quite low, and is very easy to form inclusion body, thus, the probability of getting the active protein is very low. Leading Biology using a new prokaryotic expression system, greatly increased the probability of disulfide bond rich protein expression in Escherichia coli, the expression rate is  greater than 90%. Service process Service Items Time(weeks) Protein analysis and codon optimization Before project start Gene synthesis 2-4 Vector construction 1 E.coli expression strains transformation and screening 2 Target protein inducible expression ...
  • Protein Expression in Mammalian Cells
    Application list of expression system  ProkaryoticEukaryoteEukaryoteEukaryoteAdvantage fast; widely used. fast; partial post-translational modification soluble protein; translation modification similar to mammalian system better post-translation modification; Disadvantage Non-human-derived glycosylation; Long cycle Lack of partial glycolytitization Recommended Expressions cytokines; small molecular weight proteins; cytoplasmic proteins; toxic proteins; transmembrane proteins; secretion proteins; kinases ExpressionCell l...
  • Protein Expression in Baculovirus
    Insect baculovirus expression vector system introduction Insect baculovirus expression vector system (BEVS) is a system which baculovirus  worked as a vector of foreign genes, by infect insect cells or insect larvaes to produce exogenous proteins. It is an important part of eukaryotic expression system. Advantages of insect baculovirus expression system: 1). Safe 2). Large capacity of carrying foreign genes 3). High expression level of exogenous protein 4). The expressed eukaryotic gene products can be properly folded and modified, thus have good bioactivity. 1.Advantages of insect expression system compared to mammalian cell expression system: 1). The culture conditions are relatively simple and do not require the CO2 2). The cost is relatively reasonable 3). Not easy contaminated by bacteria 4). High protein content The technical advantages of Leading Biology 1. We use classic Bac-to-Bac technology to create a fast, simple and efficient baculovirus expression platform. The technique can produce recombinant virus quickly, and do not require tedious plaque assay, ...
  • Protein Expression in Yeast
    Pichia pastoris expression system introduction The expression system of Pichia pastoris is a rapid developed and widely used eukaryotic expression system. It is a methanol nutrition yeast and have two Alcohol oxidase (AOX) coding genes AOX1 and AOX2. The two sequences are similar to each other, but the AOX1 gene is strictly regulated by methanol induction and regulation. When methanol is the only carbon source, the AOX1 promoter can be induced by methanol and initiate the expression of ethanol oxidase, which can be metabolized by methanol. The plasmid containing AOX1 promoter can be used to promote the expression of target gene which encoding foreign protein. Characteristics of expression strain GS115: Both AOX1 and AOX2 gene could express AOX protein (Alcohol Oxidase), high methanol utilization, close to the wild type, belong to methanol rapid use type (Mut+). KM71: Most AOX1 gene were knocked out,only AOX2 coding the AOX protein, the rate of using methanol is slow, belong to methanol slow use type (Muts). different integration of exogenous gene will cause different phenotypes of expression strains: ...
  • Protein Expression in Bacillus subtilis
    Bacillus subtilis expression system introduction 1. Simple cell wall composition, the secreted protein products don’t contain endotoxin. 2. The ability to produce a large number of extracellular/target proteins, simple recovery and purification. 3. Having a high efficient secretory signal peptide and molecular chaperone system, could achieve high efficient secretory of target protein, avoid the formation of inclusion bodies. Advantages of the Bacillus subtilis expression service in Leading Biology 1. Having a variety of Bacillus subtilis expressing vectors, such as pHT101 and pHT254. 2. Having many different expressions  hosts, such as intracellular expression host AS1, 1012 and 168, secretory expression host WB800N etc., could fulfill different requirements of customers. 3. Could prepare high efficient competent Bacillus subtilis. Service process Classic case An influenza virus capsid protein, molecular weight 100kD, the customer provides genetic information, after genetic optimization, the vector was constructed and expressed in Bacillus subtilis 168 and 1012: ...
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