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Location: Home > Custom Services > Antibody Discovery Services > Mouse Polyclonal Antibody Service

Mouse Polyclonal Antibody Service

Date: 2018-08-27 Author: Leading Biology Click: 2332

Polyclonal Antibody Services

Our mouse polyclonal antibodies are available in a wide variety of custom configurations and standard packages to optimize research experience. A polyclonal antibody from Leading Biology is flexible, reliable and easy to use which enables good results for any experiment. 


Service Package 

(at least 5 mice)


Service Items

Time

(weeks)

Peptide package

Protein package

Peptide

Small

Molecule

Phosphate/Methyl/Acetyl-specific

Purified protein

Expression plasmid

Gene template

Peptide design & synthesis

Peptide design

2-3

Free, Optional

 

 

 

Peptide synthesis

 

 

 

 

Conjugation

 

 

 

Prokaryotic proteins preparation

cDNA synthesis

2-4

 

 

 

 

 

Need further inquiry

Vector construction

 

 

 

 

 

Protein expression & purification

 

 

 

 

Immunization

Immune 2 mice

8-10

Elisa test

Purification and detection

Affinity purification

1-2

 

 

Antigen specific affinity purification

 

 

 

 

Total lead time(weeks)

12-14

8-9

11-12

14-16


Note:

1.Because the laboratory animals are  relatively fragile, and the preparation process is complex, to prevent experimental accidents, the number of mice for polyclonal antibody preparation project is at least five, each antigen at least immune 3 mice;

2.We use SPF level mice in the project, and Infectious antigen is strictly prohibited, the customers should acknowledge us if there are specific problems of the antigen, if the customers hiding their antigen information deliberately, and cause significant financial losses of the company, we will reserve the right to the legal responsibility;

3.For mouse polyclonal antibody services, if the customer want antigen specific affinity purification rather than normal specific affinity purification, the price increases 200$;

4. The prices listed above are for reference only, the exact price needs to be decided according to the actual situation of the project.


Classic case 

(Mouse Polyclonal Antibody Service)


Customer Provide

Protein information and antibody requirements


Process

1. Peptide design, synthesis and conjugation (1-2 weeks)

2. Feeding and immunization of 5 mice/antigen (4 immunizations, 8 weeks)

3. Blood collection

4. Elisa test, WB test (1 week)

5. Purification and delivery (1-2 weeks)


Products

(each antigen 5 mice)

1. 0.5-1mL negative serum

2. 5-10mL purified positive serum,ELISA titer was above 1:60,000

3. Complete lab report, remaining antigens

Preparation time: 2-3 months


Successful case

Publication: Cancer Science

IF:3.054

Title: Phloroglucinol suppresses metastatic ability of breast cancer cells by inhibition of epithelial-mesenchymal cell transition

Name: FN1; mouse polyclonal antibody

Species: human Fibronectin

Type: Mouse polyclonal antibody


F1. Phloroglucinol suppresses mesenchymal traits of basal type breast cancer cells. (a, b) Western blot (a) and immunocytochemical analysis (b) for EMT markers in basal type breast cancer cells after treatment with vehicle or phloroglucinol (10, 30 or 50 lM). (c) Western blot analysis for EMT master regulators after treatment with vehicle or phloroglucinol (10, 30 or 50 lM). (d) Western blot analysis for EMT markers in basal type breast cancer cells after treatment with siRNA targeting SLUG. (e) Migration and invasion assay after treatment with siRNA targeting SLUG in basal type breast cancer cells. b-actin was used as a loading control.



Service details


Antigen Forms

Polyclonal antibodies can be generated against both soluble and non-soluble recombinant proteins, fusion proteins, gel strips, existing peptide, etc. Live or toxic substances cannot be accepted.


Antigen Quantity

We recommend sending 2-3 mg of the antigen for a rabbit or chicken project and 5-7 mg for a goat project. Extensions or immunochemistry services may require additional antigen.


Antigen Concentration

Leading Biology can work with most protein concentrations as long as this information is provided. 1 mg/ml is a good target range, but higher concentrations are welcome. We consider 0.25 to .5 mg/ml a minimum concentration in most cases to ensure maximum protein volumes in each immunization.


Considerations for Gel Bands

Immunizing with gel bands works well for generating polyclonal antibodies against a purified protein. If providing a gel strip, please stain with coomassie blue, de-stain, rinse and cut out the strip containing the target protein. Please avoid freezing or homogenizing the gel strip so that we can aliquot appropriate quantities of the protein for each immunization. With gel bands in particular, it is important to maintain a higher concentration of the protein as outlined above.


Shipping Antigens to Leading Biology

Please ship the antigen in a Styrofoam box with ice packs (dry ice isn’t necessary) via FedEx, UPS or DHL Overnight to the address listed on our Order Form. 


Related Services

Rapid Antibody Service

Chicken Polyclonal Antibody Service

Rat Polyclonal Antibody Service

Mouse Polyclonal Antibody Service

Rabbit Polyclonal Antibody Service

Polyclonal Antibody Serum Preparation


Why Leading Biology?


With over 5 years of experience in custom antibodies, we offer numerous benefits for developing your custom antibody:


• Our industry leading titer guarantees of 1:50,000 eliminate the risk of not obtaining antibodies against peptide antigens.

• Our economies of scale allow us to pass cost savings to you and help maximize your budget.
• 100% of services are transparent throughout.
• Antibodies from our facility have been cited in many published research papers.
• By outsourcing antibody production and characterization needs to us, you can spend more time focused on your research.

• Our ideal location in California ensures that animals benefit from outdoor facilities and mild year-round temperatures.

• Confidentiality is a top priority for all projects. Antibodies that we manufacture belong to you and will not be commercialized.


Contact Information

Please obtain a quote before ordering, and refer to the quote number when you place an order.
Orders are typically confirmed within 12 hours.
Have a Question? Email us info@leadingbiology.com
Order Products: Order Related Products
By Phone: 1-661-524(LBI)-0262 (USA)


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