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Location: Home > Custom Services > Antibody Discovery Services > Anti-hapten Antibody Production Service

Anti-hapten Antibody Production Service

Date: 2018-08-27 Author: Leading Biology Click: 2437

Introduction

The so-called hapten is some small molecular weight or weak immunogenicity compounds and molecules, due to their small space structures, they can't form the effective epitopes which could be recognized by antibodies, this kind of hapten antibodies preparation is difficult because it is hard to cause an immune response in immune animals.


Leading Biology has a professional technical team in biology, chemistry and polymer science, by using various physical and chemical techniques to do the artificial modification of the small molecule hapten, greatly enhances the immunogenicity while preserving the characteristic epitope of the hapten, improves the preparation process of hapten antibody. On the one hand, we have a variety of carrier proteins on market, they can be flexibly combined according to the antigenic properties of the customer, on the other hand, we still do molecular design and modification on the existing carrier, made them could adapt the characteristics of all kinds of immunogen, improve the application range of carrier.


Service Package


Service Items

Time (weeks)

Polyclone

Hybridoma/Monoclonal

Molecular design \ Convertion \Conjugation

Molecular design and convertion

-2

 

 

Conjugation

Immunization

Immune 2 rabbits

-8

 

 

Immune 5 mice

 

 

ELISA test

Cell Fusion & Screening

1.5

 

 

Subcloning

1

 

 

Ascites production

(Inject 5 mice)

1

 

 

Purification and detection

Affinity purification

-1

Antigen specific affinity purification

 

 

 

 

Total lead time(months)

2-3

3-5

3-4

3-5



Classic case 

(Twice Transmembrane antibody service)


Customer Provide

Target antigen small molecule


Process

1. Molecular structure analysis, active group transformation and vector conjugation

2. Immunization of 5-6 Balb/c mice.

3. ELISA/Western-blot test to monitor the antibody titer and specificity in mice serum, according to ELISA and WB test result, screening mice.

4. Cell fusion: fusing with the myeloma cells SP2/0.

5. Positive screening: Using ELISA test to screening the antibody positive hybridoma clone.

6. Subclone: Using limited dilution to produce subclone from a positive clone. According to customers’ requirements, we also could screen out the monoclonal cell line which could produce Ig antibodies firmly (extra charge).

7. Ascites preparation: Using mice celiac inoculation to produce the ascites of hybridoma products.

8. Antibody purification: Purify the secretory antibodies from 2 monoclonal hybridoma cell lines.


Products 

(each antigen, 5 mice)

1. 5mg polypeptide

2. 2 immunogen specific monoclonal hybridoma cell lines.

3. Each cell line provides 10ml(1mg/ml)purified antibody, ELISA  titer was above 1:50,000.WB test positive.

4. If customers require, we could provide: prepared ascites (titer 1:500~1000, extra charge), hybridoma cell culture supernatant (for WB test).

5. Complete lab report, remaining antigens.


Preparation time

4-5 months


         Result

Publication: PLoS One.        

IF:3.05

Title: Wnt signaling activates Shh signaling in early postnatal intervertebral discs, and re-activates Shh signaling in old discs in the mouse.

Name: Anti-chondroitin sulfate antibody

Species: mouse

Type: Mouse monoclonal antibody

F1. The antibody staining Chondroitin Sulfate in 16 µm thick transverse sections of Apteronotus leptorhynchus whole body tissue by Immunohistochemistry (Frozen sections).Tissue was fixed in 2% paraformaldehyde, blocked with 3% sheep serum, 1% BSA, 1% teleostean gelatine in TBS for 1 hour at 24°C and then incubated with ab11570 at a 1/50 dilution for 18 hours at 4°C. The secondary used was an Alexa-Fluor 546 conjugated goat anti-mouse polyclonal used at a 1/200 dilution.



Related Services

Anti-Membrane Protein Antibody Discovery

Anti-autologous Antigen Antibody Production Service

Antibody Production by Liposome Immunization

Anti-hapten Antibody Production Service

Membrane Protein Antibody Production


Why Leading Biology?


With over 5 years of experience in custom antibodies, we offer numerous benefits for developing your custom antibody:


• Our industry leading titer guarantees of 1:50,000 eliminate the risk of not obtaining antibodies against peptide antigens.

• Our economies of scale allow us to pass cost savings to you and help maximize your budget.
• 100% of services are transparent throughout.
• Antibodies from our facility have been cited in many published research papers.
• By outsourcing antibody production and characterization needs to us, you can spend more time focused on your research.

• Our ideal location in California ensures that animals benefit from outdoor facilities and mild year-round temperatures.

• Confidentiality is a top priority for all projects. Antibodies that we manufacture belong to you and will not be commercialized.


Contact Information

Please obtain a quote before ordering, and refer to the quote number when you place an order.
Orders are typically confirmed within 12 hours.
Have a Question? Email us info@leadingbiology.com
Order Products: Order Related Products
By Phone: 1-661-524(LBI)-0262 (USA)

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