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Location: Home > Custom Services > Antibody Discovery Services > Anti-autologous Antigen Antibody Production Service

Anti-autologous Antigen Antibody Production Service

Date: 2018-08-28 Author: Leading Biology Click: 2848

Introduction

The preparation of anti-autologous antigen antibody refers to the use of normal proteins and small molecules which exists in the normal immune animals, or proteins and small molecules from other species which have high sequence conservation or structural consistency with natural immune animals as an antigen or hapten to immune these animals themselves to prepare the corresponding antibodies. Since the immunogens exist in natural immune animals, the animal immune system is hard to start the cleaning reaction (immune reaction) as a reaction to exogenous substances, this reaction is regulated by the self-protective mechanism, because once these foreign antigens were considered as an immunogen, it will cause the immune system to attack the normal proteins which have similar structures, induce autoimmune diseases. Therefore, the immunogenicity of these self-antigens are often very low, if not consider replacing the host species, it is difficult to prepare, but sometimes, due to the limitation of experimental conditions, we cannot make this choice, so the preparation of anti-autologous antigen antibody is very difficult.


Rely on the experience of the animal model technology team, Leading Biology first developed the method of target antigen gene knockout to prepare antibodies. Using this method, we can avoid the interference of endogenous immunogens, on the one hand, on the other hand, the rotation effect of antigen has been clearly demonstrated, when to harvest the gene knockout animals and autoimmune antibodies produced by the animals,  we can also observe the target immunogen injection effect on the body level, which is very suitable for a large research project aimed at a specific class of antigen.


Service Package


Service Items

Time (months)

Polyclone

Hybridoma/Monoclonal





Animal Model Construction

Plasmid construction

6-8

Target Gene Knock out or Cut off by Crisper/Cas9

Screening embryos injected with transplant

Progeny identification and propagation of conservation

Molecular design \ convertion \conjugation

Peptide design

-2

 

 

Peptide or protein synthesis

 

 

 

Immunization

Immune 2 mice

-8

 

 

Immune 5 mice

 

 

ELISA test

Cell Fusion & Screening

1.5

 

 

Subcloning

1

 

 

Ascites production

(Inject 5 mice)

1

 

 

Purification and detection

Affinity purification

-1

Antigen specific affinity purification

 

 

 

 

Total lead time(months)

8-10

8-12


Note:

By now, since mice are the common model for transgenic animals, this process can only be used for mice polyclonal antibody preparation.


Related Services

Anti-Membrane Protein Antibody Discovery

Anti-autologous Antigen Antibody Production Service

Antibody Production by Liposome Immunization

Anti-hapten Antibody Production Service

Membrane Protein Antibody Production


Why Leading Biology?

With over 5 years of experience in custom antibodies, we offer numerous benefits for developing your custom antibody:


• Our industry leading titer guarantees of 1:50,000 eliminate the risk of not obtaining antibodies against peptide antigens.

• Our economies of scale allow us to pass cost savings to you and help maximize your budget.
• 100% of services are transparent throughout.
• Antibodies from our facility have been cited in many published research papers.
• By outsourcing antibody production and characterization needs to us, you can spend more time focused on your research.

• Our ideal location in California ensures that animals benefit from outdoor facilities and mild year-round temperatures.

• Confidentiality is a top priority for all projects. Antibodies that we manufacture belong to you and will not be commercialized.


Contact Information

Please obtain a quote before ordering, and refer to the quote number when you place an order.
Orders are typically confirmed within 12 hours.
Have a Question? Email us info@leadingbiology.com
Order Products: Order Related Products
By Phone: 1-661-524(LBI)-0262 (USA)

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