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Insect Baculovirus Expression Vector SystemInsect baculovirus expression vector system introduction
Insect baculovirus expression vector system (BEVS) is a system in which baculovirus worked as a vector of foreign genes, by infect insect cells or insect larvae to produce exogenous proteins. It is an important part of the eukaryotic expression system.
Advantages of insect baculovirus expression system
1). Safe
2). Large capacity of carrying foreign genes
3). High expression level of exogenous protein
4). The expressed eukaryotic gene products can be properly folded and modified, thus have good bioactivity.
Advantages
Compared to mammalian cell expression system:
1). The culture conditions are relatively simple and do not require the CO2
2). The cost is relatively reasonable
3). Not easy contaminated by bacteria
4). High protein content
The technical advantages of Leading Biology
1. We use classic Bac-to-Bac technology to create a fast, simple and efficient baculovirus expression platform. With the technique, we can produce recombinant virus quickly, and do not require tedious plaque assay, which greatly improves the efficiency.
2. The production cycle is short and the flux is large. With the technique, we can achieve the process of both small scale cell culture and large scale fermentation, and the protein products can even reach the level of 50mg.
3. We provide free virus ion amplification and storage services, and the shelf-life can as long as half a year.
Service process
Classic case
The expression of Rsignal1 recombinant protein in Sf9 cells.
After codon optimization, the Rsignal1 target gene is constructed on the insect expression vector, then transfected with Sf9 cells to get P1 baculovirus. Through a series of virus amplification and optimal MOI exploration and high yield affinity purification, finally obtained the high-quality Rsignal1 protein.
Fig1.P1 expression test Fig.2 The optimal MOI test
Fig. 3 Purification test Fig. 4 Final product
Introduction of insect baculovirus expression vector
Common expression vectors and their features
Vector
Features
pFastBac1
Strong AcMNPV polyhedron(PH) promoter for high level protein
expression
Large multiple cloning site for simplified cloning
pFastBacHT
Strong polyhedron(PH) promoter for high-level protein
expression
N-terminal 6His tag for purification of recombinant
fusion protein using metal-chelating resin and aTEV protease cleavage site for
removal of the 6His tag following protein purification
Vector supplied in 3 reading frames for simplified cloning
pFastVacDual
Two strong baculovirus promoters(PH and p10) to allow simultaneous expression of two
proteins
Two large multiple cloning sites for simplified cloning
Common expression cells and their features
Cell
Sorce
Application
Sf9
The Sf9 cell line is a clonal isolate derived from IPLBSF21-AE (Sf21), and are
originated from the ovary tissue of Spodoptera frugiperda chrysalis.
Suitable for transfection, purification, production of high titer virus, cell plating and recombinant protein
expression.
Sf21
Sf21 cell is also a clonal isolate derived from the IPLBSF-21 cell line and
also are originated from the ovary tissue of the pupae of the Spodoptera
frugiperda.
Also suitable for transfection, purification, production of high titer
virus, cell plating and recombinant protein expression. Sometimes, Sf21 cells
may have a higher protein expression rate than Sf9 cells
Hi5
Hi5 cell lines discovered by Boyce Thompson Institute during the study
of plants, they are originated from cabbage looper ovary cells and
Trichoplusiani cells
Hi5 cells could express high quality recombinant virus. It is also widely used for
transfection and plaque purification, moreover, the Hi5 cell line is more
suitable for the expression of secretory recombinant protein.
S2
S2 are late fruit fly embryo cells. Most of them are female tetraploid
cells, and there are also some diploid cells.
It is suitable for the expression of recombinant
protein
Related Services
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Protein Expression in Mammalian Cells
Protein Expression in Baculovirus
Protein Expression in Bacillus subtilis
Why Leading Biology?
At Leading Biology, we custom protein purification design for every single protein to ensure the production and recovery rate as high as possible.
Working with us, you will get stability, and it means a reliable partner to help streamline your R&D process.
Working with us, you will get the guaranteed service to accommodate your requirements.
- Innovative configurations of chromatography columns custom tailored to each protein
- Vigorous quality control system to ensure the required quality and reproducibility
- Production capacity of up to tens of grams
- Flexible scale-up protein production
- Competitive price with fast turnaround time
Contact Information
Please obtain a quote before ordering, and refer to the quote number when you place an order.
Orders are typically confirmed within 12 hours.
Have a Question? Email us info@leadingbiology.com
Order Products: Order Related Products
By Phone: 1-661-524(LBI)-026d2 (USA)
No | Headline | Click | Author | Date |
1 | Ubiquitination | 1208 | Leading Biology | 2020-06-24 |
2 | Acetylation | 776 | Leading Biology | 2020-06-23 |
3 | Single Molecule Protein Detection | 1021 | Leading Biology | 2020-06-22 |
4 | Phosphorylation | 1045 | Leading Biology | 2020-06-22 |
5 | Methylation | 1123 | Leading Biology | 2020-06-22 |
6 | Protein Detection and Immunoassay | 1154 | Leading Biology | 2020-06-17 |