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PRDX2 Antibody (C-term)

AMR09501G Product Datasheet Protocol

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AMR09501G		100 μl / $495

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Leading Biology Inc.

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PRDX2 Antibody (C-term)

Brand	Leading Biology	Catalog Number	AMR09501G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality PRDX2 antibody (C-term).
Molecular Weight	21892 Da
Cellular Localization	Antigen Cellular Localization: Cytoplasm.
Host	Rabbit
Species Reactivity	Human, Mouse
Immunogen	169-198 aa
Target	This PRDX2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 169-198 amino acids from the C-terminal region of human PRDX2.
Isotype	Rabbit Ig
Symbol	NKEFB, TDPX1
GeneID	7001
UniProt ID	P32119
Function	Involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system. It is not able to receive electrons from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2).
Summary	PRDX2 is a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. This protein may play an antioxidant protective role in cells, and may contribute to the antiviral activity of CD8(+) T-cells. This protein may have a proliferative effect and play a role in cancer development or progression. The crystal structure of this protein has been resolved to 2.7 angstroms.
Form	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, IHC-P, IF, E
Dilution	WB~~1:1000 IF~~1:10~50 IHC-P~~1:50~100
Synonyms	Peroxiredoxin-2, Natural killer cell-enhancing factor B, NKEF-B, PRP, Thiol-specific antioxidant protein, TSA, Thioredoxin peroxidase 1, Thioredoxin-dependent peroxide reductase 1, PRDX2, NKEFB, TDPX1
Images	Western blot analysis of lysates from mouse heart and rat heart tissue lysate (from left to right), using PRDX2 Antibody (C-term) ( AMR09501G). AMR09501G was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane. Fluorescent confocal image of Hela cell stained with PRDX2 Antibody (C-term)( AMR09501G).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PRDX2 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 μg/ml, 10 min).PRDX2 immunoreactivity is localized to Cytoplasm significantly. Western blot analysis of PRDX2 Antibody (C-term) (Cat. AMR09501G) in MDA-MB231,293 cell line and mouse brain tissue lysates (35ug/lane).PRDX2 (arrow) was detected using the purified Pab.(2ug/ml)
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