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TPI1 Antibody (C-term)

APR10520G Product Datasheet Protocol

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APR10520G		100 μl / $495

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Leading Biology Inc.

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TPI1 Antibody (C-term)

Brand	Leading Biology	Catalog Number	APR10520G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality TPI1 antibody (C-term).
Molecular Weight	30791 Da
Host	Rabbit
Species Reactivity	Human, Mouse
Immunogen	258-286 aa
Target	This TPI1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 258-286 amino acids from the C-terminal region of human TPI1.
Isotype	Rabbit Ig
Symbol	TPI
GeneID	7167
UniProt ID	P60174
Summary	TPI1 is an enzyme, consisting of two identical proteins, which catalyzes the isomerization of glyceraldehydes 3-phosphate (G3P) and dihydroxy-acetone phosphate (DHAP) in glycolysis and gluconeogenesis.
Form	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, IHC-P, FC, E
Dilution	WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50
Synonyms	Triosephosphate isomerase, TIM, Triose-phosphate isomerase, TPI1, TPI
Images	Western blot analysis of lysates from A431, HepG2, LNCaP cell line and mouse testis, rat testis tissue lysates (from left to right), using TPI1 Antibody (C-term) ( APR10520G). APR10520G was diluted at 1:1000 at each lane.A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane. Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with TPI1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. TPI1 Antibody (C-term) (Cat. APR10520G) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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