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(Mouse) Cldn6 Antibody (C-term)

APR10975G Product Datasheet Protocol

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APR10975G		100 μl / $495

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Leading Biology Inc.

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(Mouse) Cldn6 Antibody (C-term)

Brand	Leading Biology	Catalog Number	APR10975G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality (Mouse) Cldn6 antibody (C-term).
Molecular Weight	23388 Da
Cellular Localization	Antigen Cellular Localization: Cell junction, tight junction. Cell membrane; Multi-pass membrane protein
Host	Rabbit
Species Reactivity	Mouse
Target	This Mouse Cldn6 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 186-229 amino acids from the C-terminal region of mouse Cldn6.
Isotype	Rabbit Ig
GeneID	54419
UniProt ID	Q9Z262
Function	Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium- independent cell-adhesion activity.
Summary	Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium- independent cell-adhesion activity (By similarity).
Form	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, FC, E
Dilution	FC~~1:25 WB~~1:1000
Synonyms	Claudin-6, Skullin, Cldn6
Images	Overlay histogram showing colon26 cells stained with APR10975G (red line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (APR10975G, 1:25 dilution) for 60 min at 37?C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit lgG (H+L) (1583138) at 1/400 dilution for 40 min at 37?C. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed. Western blot analysis of lysate from mouse kidney tissue lysate, using Cldn6 Antibody (C-term)( APR10975G). APR10975G was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
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