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ADORA2A Antibody (C-term)

APG01585G Product Datasheet Protocol

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APG01585G		100 μl / $495

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Leading Biology Inc.

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ADORA2A Antibody (C-term)

Brand	Leading Biology	Catalog Number	APG01585G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality ADORA2A antibody (C-term).
Molecular Weight	44707 Da
Cellular Localization	Antigen Cellular Localization: Cell membrane; Multi-pass membrane protein.
Host	Rabbit
Species Reactivity	Human
Target	This ADORA2A antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 347-381 amino acids from the C-terminal region of human ADORA2A.
Isotype	Rabbit Ig
Symbol	ADORA2
GeneID	135
UniProt ID	P29274
Function	Receptor for adenosine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.
Summary	Receptor for adenosine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.
Form	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, IHC-P, FC, E
Dilution	FC~~1:25 IHC-P~~1:25 WB~~1:1000
Synonyms	Adenosine receptor A2a, ADORA2A, ADORA2
Images	Overlay histogram showing SH-SY5Y cells stained with APG01585G (green line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (APG01585G, 1:25 dilution) for 60 min at 37?C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit lgG (H+L) (1583138) at 1/400 dilution for 40 min at 37?C. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed. APG01585G staining ADORA2A in Human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody. Western blot analysis of lysate from SH-SY5Y cell line, using ADORA2A Antibody (C-term)( APG01585G). APG01585G was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
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