> Antigen, Antibodies, ELISA, Western Blot > Primary Antibody > Polyclonal Antibodies > RBM3 Antibody (Center)Brand |
Leading Biology | Catalog Number |
AMR09701G |
Product Type |
Polyclonal Antibodies | Field of Research |
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Product Overview |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality RBM3 antibody (Center).
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Molecular Weight |
17170 Da
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Cellular Localization |
Antigen Cellular Localization:
Nucleus. Cytoplasm. Cell projection, dendrite Note=Localizes in mRNA granules in dentrites.
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Host |
Rabbit
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Species Reactivity |
Human
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Immunogen |
55-84 aa
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Target |
This RBM3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 55-84 amino acids from the Central region of human RBM3.
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Isotype |
Rabbit Ig
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Symbol |
RNPL
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GeneID |
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UniProt ID |
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Function |
Cold-inducible mRNA binding protein that enhances global protein synthesis at both physiological and mild hypothermic temperatures. Reduces the relative abundance of microRNAs, when overexpressed. Enhances phosphorylation of translation initiation factors and active polysome formation (By similarity).
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Summary |
RBM3 is a member of the glycine-rich RNA-binding protein family and a protein with one RNA recognition motif (RRM) domain. Expression of this protein is induced by cold shock and low oxygen tension.
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Form |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
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Storage & Stability |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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Applications |
WB, IHC-P, FC, E
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Dilution |
WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
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Synonyms |
Putative RNA-binding protein 3, RNA-binding motif protein 3, RNPL, RBM3, RNPL
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Images |
Anti-RBM3 Antibody (Center) at 1:1000 dilution + Jurkat whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 17 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot analysis of RBM3 antibody (Center) (Cat. AMR09701G) in NIH-3T3 cell line lysates (35ug/lane). RBM3 (arrow) was detected using the purified Pab.
RBM3 Antibody (Center) (RB18776) IHC analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RBM3 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated. |
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Specification |
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Quantity |
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Leading Biology Inc.
2600 Hilltop DR, Building G, B Suite C138
Richmond, CA, 94806
Tel: 1-661-524(LBI)-0262
Email: info@leadingbiology.com
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