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EEF1D Antibody (N-term)

Catalog # Availability Size / Price Inquiry
AMM07016G 100 μl / $495

EEF1D Antibody (N-term)

Brand

Leading Biology

Catalog Number

AMM07016G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality EEF1D antibody (N-term).

Molecular Weight

31122 Da

Cellular Localization

Antigen Cellular Localization: Isoform 2: Nucleus

Host

Rabbit

Species Reactivity

Human

Immunogen

1-30 aa

Target

This EEF1D antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human EEF1D.

Isotype

Rabbit Ig

Symbol

EF1D

GeneID

UniProt ID

Function

Isoform 1: EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP, regenerating EF-1- alpha for another round of transfer of aminoacyl-tRNAs to the ribosome.

Summary

EEF1D is a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit functions as guanine nucleotide exchange factor. It is reported that this subunit interacts with HIV-1 Tat, and thus it represses the translation of host-cell, but not HIV-1, mRNAs.

Form

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, IHC-P, FC, E

Dilution

WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50

Synonyms

Elongation factor 1-delta, EF-1-delta, Antigen NY-CO-4, EEF1D, EF1D

Images

Western blot analysis of EEF1D antibody (N-term) (Cat. AMM07016G) in Ramos cell line lysates (35ug/lane). EEF1D (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human lymph reacted with EEF1D Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

EEF1D Antibody (N-term) (Cat. AMM07016G) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specification

Quantity

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