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ARPC1A Antibody (C-term)

APR15057G Product Datasheet Protocol

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APR15057G		100 μl / $495

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Leading Biology Inc.

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ARPC1A Antibody (C-term)

Brand	Leading Biology	Catalog Number	APR15057G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality ARPC1A antibody (C-term).
Molecular Weight	41569 Da
Cellular Localization	Antigen Cellular Localization: Cytoplasm, cytoskeleton.
Host	Rabbit
Species Reactivity	Human
Immunogen	286-315 aa
Target	This ARPC1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 286-315 amino acids from the C-terminal region of human ARPC1A.
Isotype	Rabbit Ig
Symbol	SOP2L
GeneID	10552
UniProt ID	Q92747
Function	Probably functions as component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks.
Summary	ARPC1A is one of seven subunits of the human Arp2/3 protein complex. This subunit is a member of the SOP2 family of proteins and is most similar to the protein ARPC1B. The similarity between these two proteins suggests that they both may function as p41 subunit of the human Arp2/3 complex that has been implicated in the control of actin polymerization in cells. It is possible that the p41 subunit is involved in assembling and maintaining the structure of the Arp2/3 complex. Multiple versions of the p41 subunit may adapt the functions of the complex to different cell types or developmental stages.
Form	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, IHC-P, IF, FC, E
Dilution	WB~~1:1000 IHC-P~~1:10~50 IF~~1:10~50 FC~~1:10~50
Synonyms	Actin-related protein 2/3 complex subunit 1A, SOP2-like protein, ARPC1A, SOP2L
Images	Anti-ARPC1A Antibody (C-term) at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 42 kDa Blocking/Dilution buffer: 5% NFDM/TBST. Formalin-fixed and paraffin-embedded human skeletal muscle reacted with ARPC1A Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Immunofluorescence analysis of ARPC1A Antibody (C-term) with hela cells. 0.025 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.
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