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ACAP1 Antibody (N-term)

APR14781G Product Datasheet Protocol

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APR14781G		100 μl / $495

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Leading Biology Inc.

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ACAP1 Antibody (N-term)

Brand	Leading Biology	Catalog Number	APR14781G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality ACAP1 antibody (N-term).
Molecular Weight	81536 Da
Cellular Localization	Antigen Cellular Localization: Recycling endosome membrane; Peripheral membrane protein; Cytoplasmic side
Host	Rabbit
Species Reactivity	Human, Mouse
Immunogen	96-125 aa
Target	This ACAP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 96-125 amino acids from the N-terminal region of human ACAP1.
Isotype	Rabbit Ig
Symbol	CENTB1, KIAA0050
GeneID	9744
UniProt ID	Q15027
Function	GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6) required for clathrin-dependent export of proteins from recycling endosomes to trans-Golgi network and cell surface. Required for regulated export of ITGB1 from recycling endosomes to the cell surface and ITGB1-dependent cell migration.
Summary	Catalyzes the transfer of Gal to GlcNAc-based acceptors with a preference for the core3 O-linked glycan GlcNAc(beta1,3)GalNAc structure. Can use glycolipid LC3Cer as an efficient acceptor.
Form	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, IHC-P, E
Dilution	WB~~1:1000 IHC-P~~1:50~100
Synonyms	Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1, Centaurin-beta-1, Cnt-b1, ACAP1, CENTB1, KIAA0050
Images	ACAP1 Antibody (N-term) (Cat. APR14781G) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the ACAP1 antibody detected the ACAP1 protein (arrow). ACAP1 Antibody (N-term) (Cat. APR14781G) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the ACAP1 antibody detected the ACAP1 protein (arrow). ACAP1 Antibody (N-term) (Cat. APR14781G)immunohistochemistry analysis in formalin fixed and paraffin embedded human spleen tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ACAP1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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