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DDA Antibody

Catalog # Availability Size / Price Inquiry
APR06721G 0.1 mg / $695

DDA Antibody

Brand

Leading Biology

Catalog Number

APR06721G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality DDA Antibody.

Molecular Weight

11835 Da

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Target

DDA1;

Isotype

IgG

Symbol

C19orf58, PCIA1

GeneID

UniProt ID

Function

May be involved in ubiquitination and subsequent proteasomal degradation of target proteins. Component of the DDD- E2 complexes which may provide a platform for interaction with CUL4A and WD repeat proteins.

Summary

DDA Antibody: DDA1 (DET1 and DDB1 associated 1), along with DET1 and DDB1 and a member of the UBE2E group of canonical ubiquitin-conjugating enzymes, comprise DDD-E2 complexes, which interact with multiple ubiquitin E3 ligases. One of these E3 ligases is Cul4-containing E3 ligase complex CRL4. Cells depleted of DDA1 spontaneously accumulate double-stranded DNA breaks in a similar fashion as Cul4A-, Cul4B-, or WDR23-depleted cells, suggesting that DDA1 interacts with the CRL4 complex and may be involved in the ubiquitination and subsequent proteasomal degradation of target proteins.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, IHC-P, IF, E

Images

Western blot analysis of DDA1 in mouse heart tissue lysate with DDA1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide

Immunohistochemistry of DDA1 in mouse heart tissue with DDA1 antibody at 5 μg/mL.

Immunofluorescence of DDA1 in mouse heart tissue with DDA1 antibody at 20 μg/mL.

Specification

Quantity

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