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CD9 Antibody (Center)

Catalog # Availability Size / Price Inquiry
APR04023G 100 μl / $495

CD9 Antibody (Center)

Brand

Leading Biology

Catalog Number

APR04023G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality CD9 Antibody (Center).

Molecular Weight

25416 Da

Cellular Localization

Antigen Cellular Localization: Membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein

Host

Rabbit

Species Reactivity

Human

Immunogen

115-145 aa

Target

This CD9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 115-145 amino acids from the Central region of human CD9.

Isotype

Rabbit Ig

Symbol

MIC3, TSPAN29

GeneID

UniProt ID

Function

Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion.

Summary

CD9 is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This protein is a cell surface glycoprotein that is known to complex with integrins and other transmembrane 4 superfamily proteins. It can modulate cell adhesion and migration and also trigger platelet activation and aggregation. In addition, the protein appears to promote muscle cell fusion and support myotube maintenance.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

IF, WB, FC, E

Dilution

IF~~1:25 WB~~1:1000 FC~~1:10~50

Images

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized Hela (Human Cervical epithelial adenocarcinoma cell line) cells labeling Pdx1 with APR04023G at 1/25 dilution, followed by Dylight? 488-conjugated goat anti-rabbit IgG (NK179883) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing membrane staining on Hela cell line. The nuclear counter stain is DAPI (blue).

Anti-CD9 Antibody (Center)at 1:2000 dilution + MCF-7 whole cell lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 25 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of lysates from Hela, MCF-7 cell line (from left to right), using CD9 Antibody (Center)(APR04023G). APR04023G was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.

Specification

Quantity

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