>
Antigen, Antibodies, ELISA, Western Blot >
Immunoassay Kits >
ELISA Kit >
Human AKT1/RAC-alpha serine/threonine-protein kinase ELISA KitBrand |
Leading Biology | Catalog Number |
EK00180E |
Product Type |
ELISA Kit | Field of Research |
|
Product Overview |
EK00180E is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (RAC-alpha serine/threonine-protein kinase) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing RAC-alpha serine/threonine-protein kinase The ELISA analytical biochemical technique of the EK00180E kit is based on RAC-alpha serine/threonine-protein kinase antibody-RAC-alpha serine/threonine-protein kinase antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect RAC-alpha serine/threonine-protein kinase antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, RAC-alpha serine/threonine-protein kinase. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV (%).
|
||
Cellular Localization |
Cytoplasm Nucleus Cell membrane Nucleus after activation by integrin-linked protein kinase 1 (ILK1). Nuclear translocation is enhanced by interaction with TCL1A. Phosphorylation on Tyr-176 by TNK2 results in its localization to the cell membrane where it is targeted for further phosphorylations on Thr-308 and Ser-473 leading to its activation and the activated form translocates to the nucleus. Colocalizes with WDFY2 in intracellular vesicles (PubMed:16792529).
|
||
Assay Type |
Sandwich
|
||
Detection Method |
Spectrophotometrical
|
||
Reactivity |
Human
|
||
Sample Type |
This immunoassay kit allows for the in vitro quantitative determination in Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.
|
||
Sensitivity |
0.195 ng/mL
|
||
Assay Range |
0.78-50 ng/mL
|
||
Kit Components |
Assay Plate, Standard, Sample Diluent, Assay Diluent A&B, Detection Reagent A&B, Wash Buffer, Substrate, Stop Solution.
|
||
Other Reagents Required |
Deionized or distilled water
|
||
Gene Name |
AKT1
|
||
MIM |
|||
UniProt ID |
|||
Function |
AKT1-specific substrates have been recently identified, including palladin (PALLD), which phosphorylation modulates cytoskeletal organization and cell motility; prohibitin (PHB), playing an important role in cell metabolism and proliferation; and CDKN1A, for which phosphorylation at 'Thr-145' induces its release from CDK2 and cytoplasmic relocalization. These recent findings indicate that the AKT1 isoform has a more specific role in cell motility and proliferation. Phosphorylates CLK2 thereby controlling cell survival to ionizing radiation.
|
||
Form |
ELISA Kits |
||
Storage & Stability |
Store at +4°C. Please refer to protocols.
|
||
Applications |
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
|
||
Synonyms |
Protein kinase B,PKB,Protein kinase B alpha,PKB alpha,Proto-oncogene c-Akt,RAC-PK-alpha,PKB,RAC
|
||
Images |
Standard curve of the product EK00180E. |
||
Specification |
|||
Quantity |
|
Select | Brand | Catalog No. | Product Name | Pack Size | Type | Field of Research | Specification | Quantity | Price(USD) | |
1 | Leading Biology | EK00487E | Human DCN/Decorin ELISA Kit | 96T | ELISA Kit |
|
$645.00 | Add Ask | ||
2 | Leading Biology | EK00726E | General HS/Heparan sulfate ELISA Kit | 96T | ELISA Kit |
|
$745.00 | Add Ask | ||
3 | Leading Biology | EK00038E | General E2/Estradiol ELISA Kit | 96T | ELISA Kit |
|
$545.00 | Add Ask | ||
4 | Leading Biology | EK0003L | Exendin-4 (Exenatide) ADA | 96T | ELISA Kit |
|
$895.00 | Add Ask | ||
5 | Leading Biology | EK00003E | General Testosterone/Testosterone ELISA Kit | 96T | ELISA Kit |
|
$545.00 | Add Ask | ||
6 | Leading Biology | EK00793E | General GSH/Glutathione ELISA Kit | 96T | ELISA Kit |
|
$745.00 | Add Ask |
Complete this form and click send to ask us a question, request a quote or simply say hello.
You have 0 item in your cart
You have 0 item in your inquiry list