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Location: Home > Antigen, Antibodies, ELISA, Western Blot > Other Immune Related Reagents > BCA Protein quantification kit

BCA Protein quantification kit

Catalog # Availability Size / Price Inquiry
PK001-01 BCA reagent A:100 ml; BCA reagent B:3 ml; BSA standards (2 mg/ml):2 ml / $400

BCA Protein quantification kit

Brand

Leading Biology

Catalog Number

PK001

Product Type

Other Immune Related Reagents

Field of Research

Product Overview

The BCA protein quantitative kit is developed according to the BCA (bicinchoninic acid) method, which is one of the two most commonly used methods for protein concentration detection in the world at present. This kit is the principle of the peptide bond structure of the protein molecules in alkaline environment and Cu 2+ can generate complex, Cu 2+ was reduced to Cu +, and BCA reagent can be sensitive to specific combined with Cu +, stable color of complex formation, and maximum light absorption at 562 nm, the compound color depth is proportional to the concentration of protein, can according to the size of the absorption value to determine the content of protein. This product introduces two testing schemes. In the two schemes, the test tube detection scheme requires a large volume of protein samples (0. 1 ml), and the ratio between the samples used in the scheme and the working solution is 1:20 (v/v), so as to minimize the impact of interfering substances. MPP solution samples more convenient operation, and need of protein samples (10-25 ?l) with minor volume, however, due to the use of samples and the ratio between the working liquid for 1:8 (v/v), so this method is compared with the test tube method, overcoming the influence of interfering substances on the determination results in low flexibility and low detection concentration of protein samples as a sensitive test tube method. The kit contains bovine serum albumin (BSA) solution as the standard protein solution, and the determination range is 20-2000 ?g/ml.

Kit Components

BCA reagent A: 100 ml BCA reagent B: 3 ml BSA standards (2 mg/ml): 2 ml

Form

Kit

Storage & Stability

BCA reagent A and BCA reagent B were stored at room temperature, and BSA standards were stored at -20°C.

Applications

The kit contains bovine serum albumin (BSA) solution as the standard protein solution, and the determination range is 20-2000 ?g/ml.

Images

Specification

Quantity

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