Brand |
Leading Biology | Catalog Number |
MQ002 |
Product Type |
DNA Polymerase | Field of Research |
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Product Overview |
This product is a recombinant Taq DNA polymerase modified by antibody, suitable for Hot Start PCR. At room temperature, the activity of Taq DNA polymerase is blocked by antibodies, thus inhibiting the non-specific amplification caused by the non-specific annealing of primers or the dimer of primers at room temperature. When the amplification reaction system was heated to 95°C and heated for 2min, the polymerase activity was restored due to the denaturation of Taq enzyme antibody. Therefore, no special deactivation treatment was required, and it could be used under conventional PCR reaction conditions.
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Activity |
One unit is the amount of enzyme that will incorporate 10 nmol of dNTP into acid-insoluble products in 30 minutes at 74°C with activated salmon sperm DNA as the template-primer.
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Form |
Liquid |
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Concentration |
5 U/μL |
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Storage & Stability |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 24 months at -20°C. Avoid repeated freeze-thaw cycles.
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Storage Buffer |
Tris-HCl (pH8. 0) 20 mM
KCl 100 mM
EDTA 0. 1 mM
DTT 1
Tween 20 0. 5%
Nonidet P-40 0. 5%
Glycerol 50%
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Applications |
PCR, hot start PCR, qPCR, genome PCR, TA cloning, primer extension, DNA markers
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Images |
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Specification |
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Quantity |
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Select | Brand | Catalog No. | Product Name | Pack Size | Type | Field of Research | Specification | Quantity | Price(USD) | |
1 | Leading Biology | MP002 | Kofu DNA polymerase | 250 μL | DNA Polymerase |
|
$200.00 | Add Ask | ||
2 | Leading Biology | MQ003 | Hot start TTH Polymerase | 50 μL | DNA Polymerase |
|
$0.00 | Add Ask | ||
3 | Leading Biology | MP001 | Taq DNA polymerase | 50 μL | DNA Polymerase |
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$43.00 | Add Ask | ||
4 | Leading Biology | MQ001 | Hot start CH-Taq DNA Polymerase | 50 μL | DNA Polymerase |
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$280.00 | Add Ask | ||
5 | Leading Biology | MP003 | TTH Polymerase | 50 μL | DNA Polymerase |
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$300.00 | Add Ask | ||
6 | Leading Biology | MP010 | cUracil-DNA Glycosylase | 100 μL | DNA Polymerase |
|
$610.00 | Add Ask |
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