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Location: Home > Antigen, Antibodies, ELISA, Western Blot > Primary Antibody > Polyclonal Antibodies > [KO Validated] P4HB Polyclonal Antibody

[KO Validated] P4HB Polyclonal Antibody

Catalog # Availability Size / Price Inquiry
APR17522N-1 50ul / $395
APR17522N-2 100ul / $495
APR17522N-3 200ul / $595

[KO Validated] P4HB Polyclonal Antibody

Brand

Leading Biology

Catalog Number

APR17522N

Product Type

Polyclonal Antibodies

Field of Research

Cell Biology & Developmental Biology>Cytoskeleton

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality [KO Validated] P4HB Polyclonal Antibody.

Molecular Weight

Calculated MW: 57kDa Observed MW: 57kDa

Species Reactivity

Rabbit

Immunogen

Recombinant fusion protein of human P4HB (NP_000909.2).

Cross Reactivity

Human,Mouse,Rat

Isotype

IgG

GeneID

UniProt ID

Purity

Affinity purification

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Storage Buffer

Buffer: PBS with 50% glycerol, pH7.3.

Applications

WB,IHC,IF,IP

Dilution

WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:20 - 1:100

Product Description

This gene encodes the beta subunit of prolyl 4-hydroxylase, a highly abundant multifunctional enzyme that belongs to the protein disulfide isomerase family. When present as a tetramer consisting of two alpha and two beta subunits, this enzyme is involved in hydroxylation of prolyl residues in preprocollagen. This enzyme is also a disulfide isomerase containing two thioredoxin domains that catalyze the formation, breakage and rearrangement of disulfide bonds. Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex.

Synonyms

P4HB; CLCRP1; DSI; ERBA2L; GIT; P4Hbeta; PDI; PDIA1; PHDB; PO4DB; PO4HB; PROHB; protein disulfide-isomerase

Images

Western blot analysis of extracts of various cell lines, using P4HB antibody.

Western blot analysis of extracts from normal (control) and P4HB knockout (KO) HeLa cells, using P4HB antibody.

Immunofluorescence analysis of HeLa cells using P4HB antibody.

Specification

Quantity

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