We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality Thyrotropin-Releasing Hormone Receptor 1 antibody.

46609 Da

Rabbit

Human, Mouse, Rat

Peptide CKQKPTEKAANYS, corresponding to amino acid residues 337-349 of rat Thyrotropin-releasing hormone receptor 1 (Accession Q01717). Intracellular, C-terminus.

Thyrotropin-releasing hormone (TRH), is a widely distributed tripeptide which acts as a hormone, a paracrine regulatory factor and a neurotransmitter/neuromodulator; it is expressed in many parts of the brain as well as in various peripheral tissues1,2.In the hypothalamus, TRH is secreted from the paraventricular nucleus (VPN) by TRH neurons prior to a post-translational processing from its precursor, pro-TRH. A key player in the regulation of the hypothalamus-pituitary-thyroid (HPT) axis, TRH is released into the hypophyseal portal circulation of the median eminence and reaches the pituitary, where it binds to TRH receptors, thereby stimulates the secretion of tyrothropin (thyroid-stimulating hormone; TSH). TSH, in turn, affects the thyroid to produce T4 which is subsequently converted into its biologically-active analog, T3 - a negative mediator of TRH. In the tanycytes, ependymal cells of glial origin located in close proximity to the VPN, T4 is converted to T3 by the enzyme deiodinase II (dio2) - a counterpart to deiodinase III (dio3) which conversely inactivates the above mentioned thyroid hormones, thus so indirectly promotes the production of TRHs3,4.TRH receptor 1 (TRH-R1), the only identified TRH receptor in humans, is a G protein-coupled receptor (GPCR) abundant in the anterior pituitary, neuroendocrine system, autonomic nervous system and the visceral regions of the brain2,5. Like all members of GPCRs, TRH receptor has seven transmembrane domains, and extracellular N-terminus and an intracellular C-terminal tail. TRH receptor couples Gq upon activation which in turn leads to the activation of phospholipase C5.From a behavioral aspect, it was shown that TRH-coupled TRH-R1 increases cognitive arousal by directly exciting the sleep/wake system in the lateral hypothalamic area, and inhibits seizure activity in the hippocampus by facilitating GABA release, whereas its deficiency, as observed in mutant model mice, is linked to growth retardation, depression, and anxiety-like behavior1,6,7.Abgent is pleased to offer a highly specific antibody directed against an extracellular epitope of rat TRH-R. Anti-Thyrotropin-Releasing Hormone Receptor 1 antibody (#AG1004) can be used in western blot analysis. It has been designed to recognize TRH-R from rat, mouse and human samples.

Affinity purified antibody, Liquid

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

WB

WB~~1:200-1:2000

Thyrotropin-releasing hormone receptor, TRH-R, Thyroliberin receptor, Trhr

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