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PYCR1 Antibody (C-term)

AMR09666G Product Datasheet Protocol

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AMR09666G		100 μl / $495

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Leading Biology Inc.

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PYCR1 Antibody (C-term)

Brand	Leading Biology	Catalog Number	AMR09666G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality PYCR1 antibody (C-term).
Molecular Weight	H=33,36 KDa
Cellular Localization	Antigen Cellular Localization: Mitochondrion.
Host	Rabbit
Species Reactivity	Human
Immunogen	291-319 aa
Target	This PYCR1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 291-319 amino acids from the C-terminal region of human PYCR1.
GeneID	5831
UniProt ID	P32322
Function	Housekeeping enzyme that catalyzes the last step in proline biosynthesis. Can utilize both NAD and NADP, but has higher affinity for NAD. Involved in the cellular response to oxidative stress.
Summary	This gene encodes an enzyme that catalyzes the NAD(P)H-dependent conversion of pyrroline-5-carboxylate to proline. This enzyme may also play a physiologic role in the generation of NADP(+) in some cell types. The protein forms a homopolymer and localizes to the mitochondrion.
Form	Liquid. In PBS containing 0.02% sodium azide.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, IHC-P, FC
Dilution	WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50
Synonyms	Pyrroline-5-carboxylate reductase 1, mitochondrial, P5C reductase 1, P5CR 1, PYCR1
Images	All lanes : Anti-PYCR1 Antibody (C-term) at 1:1000 dilution Lane 1: HepG2 whole cell lysate Lane 2: K562 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 33 kDa Blocking/Dilution buffer: 5% NFDM/TBST. PYCR1 Antibody (C-term) (Cat. AMR09666G) IHC analysis in formalin fixed and paraffin embedded colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PYCR1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated. PYCR1 Antibody (C-term) (Cat. AMR09666G) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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