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ACAT1 Antibody (C-term)

Catalog # Availability Size / Price Inquiry
APG01465G 100 μl / $495

ACAT1 Antibody (C-term)

Brand

Leading Biology

Catalog Number

APG01465G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality ACAT1 antibody (C-term).

Molecular Weight

H=45.2 KDa

Cellular Localization

Antigen Cellular Localization: Mitochondrion.

Host

Rabbit

Species Reactivity

Human, Mouse

Immunogen

311-349 aa

Target

This ACAT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 296-349 amino acids from the C-terminal region of human ACAT1.

Symbol

ACAT, MAT

GeneID

UniProt ID

Function

Plays a major role in ketone body metabolism.

Summary

ACAT1 is a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in the gene encoding ACAT1 are associated with the alpha-methylacetoaceticaciduria disorder,an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone.

Form

Liquid. In PBS containing 0.02% sodium azide.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, IHC-P

Dilution

IHC-P~~1:10~50 WB~~1:1000

Synonyms

Acetyl-CoA acetyltransferase, mitochondrial, Acetoacetyl-CoA thiolase, T2, ACAT1, ACAT, MAT

Images

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ACAT1 antibody (C-term) (Cat. APG01465G), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

All lanes : Anti-ACAT1 Antibody (C-term) at 1:1000 dilution Lane 1: HepG2 whole cell lysates Lane 2: mouse liver lysates Lane 3: SK-BR-3 whole cell lysates Lane 4: SW620 whole cell lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 45 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Specification

Quantity

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