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AGR2 Antibody (N-term)

AMM08751G Product Datasheet Protocol

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AMM08751G		100 μl / $495

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Leading Biology Inc.

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AGR2 Antibody (N-term)

Brand	Leading Biology	Catalog Number	AMM08751G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality AGR2 antibody (N-term).
Molecular Weight	H=20;M=20;Z=20 KDa
Cellular Localization	Antigen Cellular Localization: Secreted. Endoplasmic reticulum
Host	Rabbit
Species Reactivity	Human
Immunogen	13-42 aa
Target	This Anterior Gradient 2 (AGR2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 13-42 amino acids from the N-terminal region of human Anterior Gradient 2 (AGR2).
Symbol	AG2
GeneID	10551
UniProt ID	O95994
Function	Required for MUC2 post-transcriptional synthesis and secretion. May play a role in the production of mucus by intestinal cells (By similarity). Proto-oncogene that may play a role in cell migration, cell differentiation and cell growth.
Summary	Anterior gradient 2 (AGR2) is known as a cancer cell marker specifically up-regulated in response to depletion of serum and oxygen. AGR2 has been identified as a tumor marker in primary and secondary cancer lesions, and as a marker for detection of circulating tumor cells (CTCs). Elevated levels of AGR2 are known to increase the metastatic potential of cancer cells, but conditions leading to increased expression of AGR2 are not well understood.
Form	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, IHC-P, IF
Dilution	WB~~1:1000 IHC-P~~1:10~50 IF~~1:10~50
Synonyms	AGR2; AG2; Anterior gradient protein 2 homolog; HPC8; Secreted cement gland protein XAG-2 homolog
Images	All lanes : Anti-AGR2 Antibody (N-term)(AMM08751G) at 1/1000 dilution Lane 1: HT-29 whole cell lysates Lane 2: T47D whole cell lysates Lane 3: MCF-7 whole cell lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 19 kDa Blocking/Dilution buffer: 5% NFDM/TBST. Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with AGR2 antibody (N-term) (Cat. AMM08751G), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Fluorescent confocal image of A549 cell stained with AGR2 Antibody (N-term)( AMM08751G).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with AGR2 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 μg/ml, 10 min). AGR2 immunoreactivity is localized to Cytoplasm significantly.
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