> Antigen, Antibodies, ELISA, Western Blot > Primary Antibody > Polyclonal Antibodies > ERK3 / MAPK6 Antibody (internal region, near C-Term)Brand |
Leading Biology | Catalog Number |
APR15867G |
Product Type |
Polyclonal Antibodies | Field of Research |
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Product Overview |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality ERK3 / MAPK6 antibody (internal region, near C-Term).
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Molecular Weight |
82681 Da
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Cellular Localization |
Antigen Cellular Localization:
Cytoplasm. Nucleus. Note=Translocates to the cytoplasm following interaction with MAPKAPK5.
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Host |
Goat
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Species Reactivity |
Mouse
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Isotype |
IgG
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Symbol |
ERK3, PRKM6
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GeneID |
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UniProt ID |
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Function |
Atypical MAPK protein. Phosphorylates microtubule- associated protein 2 (MAP2) and MAPKAPK5. The precise role of the complex formed with MAPKAPK5 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPKAPK5, ERK3/MAPK6 is phosphorylated at Ser-189 and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK3/MAPK6. May promote entry in the cell cycle (By similarity).
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Summary |
Reported variants represent identical protein (NP_056621.4; NP_081694.1).
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Form |
0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin |
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Storage & Stability |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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Applications |
WB, IP, E
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Synonyms |
Mitogen-activated protein kinase 6, MAP kinase 6, MAPK 6, 2.7.11.24, Extracellular signal-regulated kinase 3, ERK-3, MAP kinase isoform p97, p97-MAPK, MAPK6, ERK3, PRKM6
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Images |
HEK293 lysate (10ug protein in RIPA buffer) overexpressing GFP-fused Mouse ERK3 probed with APR15867G (0.5ug/ml) in left panel and with anti-GFP in right panel. GFP-only exression in the first lane. Primary incubations were for 2 hours. Detected by chemiluminescence.
APR15867G (1.5ug) immunoprecipitation from lysates of MK5/ERK3 double knockout MEFs, with (third lane) and without (fourth lane) rescued MK5/ERK3 expression through retroviral transduction. The corresponding lysates (first and second lane resp.) were analyzed in parallel in this Western blot labelled with rabbit anti-Erk3 (and co-precipitation was measured using mouse anti-MK5/PRAK in the lower panel). |
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Specification |
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Quantity |
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Richmond, CA, 94806
Tel: 1-661-524(LBI)-0262
Email: info@leadingbiology.com
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