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Goat Anti-GAPDH (C Terminus) Loading Control Antibody

AMM04988G Product Datasheet Protocol

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AMM04988G		0.1 mg / $495

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Leading Biology Inc.

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Goat Anti-GAPDH (C Terminus) Loading Control Antibody

Brand	Leading Biology	Catalog Number	AMM04988G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality Goat Anti-GAPDH (C Terminus) Loading Control antibody.
Molecular Weight	36053 Da
Cellular Localization	Antigen Cellular Localization: Cytoplasm, cytosol. Nucleus. Cytoplasm, perinuclear region. Membrane. Cytoplasm, cytoskeleton. Note=Translocates to the nucleus following S- nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions.
Host	Goat
Species Reactivity	Human, Mouse, Rat, Pig
Isotype	IgG
Symbol	GAPD
GeneID	2597
UniProt ID	P04406
Function	Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D- glyceroyl phosphate. Component of the GAIT (gamma interferon- activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation.
Summary	The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome.
Form	0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, IHC, ICC, E
Synonyms	Glyceraldehyde-3-phosphate dehydrogenase, GAPDH, 1.2.1.12, Peptidyl-cysteine S-nitrosylase GAPDH, 2.6.99.-, GAPDH, GAPD
Images	AMM04988G (0.1ug/ml) staining of PFA-fixed HeLa before (top) and after (bottom) si-RNA-mediated GAPDH knock-down expresson. Primary incubation 1h at ambient temp. Detection by DyLight 488. Nuclear DAPI stain (right). AMM04988G (0.1 μg/ml) staining of HeLa lysate (control in left lane and after si-RNA-mediated GAPDH knock-down expresson in right lane) (35 μg protein in RIPA buffer). Level of knock-down relative to Actin expression level was determined by RT-PCR. Primary incubation was 1 hour. Detected by chemiluminescence. AMM04988G staining (0.01 μg/ml) of 293 lysate (RIPA buffer, 35 μg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
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