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Location: Home > Antigen, Antibodies, ELISA, Western Blot > Primary Antibody > Monoclonal Antibodies > PDHB Antibody (monoclonal) (M03)

PDHB Antibody (monoclonal) (M03)

Catalog # Availability Size / Price Inquiry
APR09008G 100 μg / $495

PDHB Antibody (monoclonal) (M03)

Brand

Leading Biology

Catalog Number

APR09008G

Product Type

Monoclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality PDHB antibody (monoclonal) (M03).

Molecular Weight

39233 Da

Host

mouse

Species Reactivity

Human

Target

PDHB (NP_000916, 250 a.a. ~ 359 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Clone

2B2

Isotype

IgG1 Kappa

GeneID

UniProt ID

Summary

The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 beta subunit. Mutations in this gene are associated with pyruvate dehydrogenase E1-beta deficiency. Alternatively spliced transcript variants have been found for this gene.

Form

Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, IHC, E

Dilution

WB~~1:500~1000

Synonyms

Pyruvate dehydrogenase E1 component subunit beta, mitochondrial, PDHE1-B, PDHB, PHE1B

Images

Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (37.84 KDa) .

PDHB monoclonal antibody (M03), clone 2B2 Western Blot analysis of PDHB expression in HeLa ( (Cat APR09008G )

Western Blot analysis of PDHB expression in transfected 293T cell line by PDHB monoclonal antibody (M03), clone 2B2. Lane 1: PDHB transfected lysate(39.2 KDa). Lane 2: Non-transfected lysate.

Specification

Quantity

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