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COX6A2 Antibody (Center)

Catalog # Availability Size / Price Inquiry
APR14314G 100 μl / $495

COX6A2 Antibody (Center)

Brand

Leading Biology

Catalog Number

APR14314G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality COX6A2 antibody (Center).

Molecular Weight

10815 Da

Cellular Localization

Antigen Cellular Localization: Mitochondrion inner membrane.

Host

Rabbit

Species Reactivity

Human

Immunogen

37-66 aa

Target

This COX6A2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 37-66 amino acids from the Central region of human COX6A2.

Isotype

Rabbit Ig

Symbol

COX6A, COX6AH

GeneID

UniProt ID

Function

This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.

Summary

Cytochrome c oxidase (COX), the terminal enzyme of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. It is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may be involved in the regulation and assembly of the complex. This nuclear gene encodes polypeptide 2 (heart/muscle isoform) of subunit VIa, and polypeptide 2 is present only in striated muscles. Polypeptide 1 (liver isoform) of subunit VIa is encoded by a different gene, and is found in all non-muscle tissues. These two polypeptides share 66% amino acid sequence identity.

Form

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, IHC-P, E

Dilution

IHC-P~~1:25 WB~~1:1000

Synonyms

Cytochrome c oxidase subunit 6A2, mitochondrial, Cytochrome c oxidase polypeptide VIa-heart, COXVIAH, Cytochrome c oxidase subunit VIA-muscle, COX VIa-M, COX6A2, COX6A, COX6AH

Images

Immunohistochemical analysis of paraffin-embedded H. skeletal muscle section using COX6A2 Antibody (Center)( APR14314G). APR14314G was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Western blot analysis of lysate from human skeletal muscle tissue lysate, using COX6A2 Antibody (Center)( APR14314G). APR14314G was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.

Western blot analysis of lysate from human heart tissue lysate, using COX6A2 Antibody (Center)(Cat. APR14314G). APR14314G was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.

Specification

Quantity

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