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GPX1 Antibody (C-term)

AMM05211G Product Datasheet Protocol

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AMM05211G		100 μl / $495

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Leading Biology Inc.

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GPX1 Antibody (C-term)

Brand	Leading Biology	Catalog Number	AMM05211G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality GPX1 antibody (C-term).
Molecular Weight	22088 Da
Cellular Localization	Antigen Cellular Localization: Cytoplasm.
Host	Rabbit
Species Reactivity	Human, Mouse, Rat
Immunogen	164-193 aa
Target	This GPX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 164-193 amino acids from the C-terminal region of human GPX1.
Isotype	Rabbit Ig
GeneID	2876
UniProt ID	P07203
Function	Protects the hemoglobin in erythrocytes from oxidative breakdown.
Summary	GPX1 encodes a member of the glutathione peroxidase family. Glutathione peroxidase functions in the detoxification of hydrogen peroxide, and is one of the most important antioxidant enzymes in humans. This protein is one of only a few proteins known in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by UGA, that normally functions as a translation termination codon. In addition, this protein is characterized in a polyalanine sequence polymorphism in the N-terminal region, which includes three alleles with five, six or seven alanine (ALA) repeats in this sequence.
Form	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	FC, WB, IHC-P, IF, E
Dilution	FC~~1:25 WB~~1:1000 IHC-P~~1:10~50 IF~~1:10~50
Synonyms	Glutathione peroxidase 1, GPx-1, GSHPx-1, Cellular glutathione peroxidase, GPX1
Images	Overlay histogram showing Hela cells stained with AMM05211G (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AMM05211G, 1:25 dilution) for 60 min at 37?C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight? 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37?C. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed. All lanes : Anti-GPX1 Antibody (C-term) at 1:2000 dilution Lane 1: rat liver lysate Lane 2: THP-1 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 22 kDa Blocking/Dilution buffer: 5% NFDM/TBST. All lanes : Anti-GPX1 Antibody (C-term) at 1:2000 dilution Lane 1: THP-1 whole cell lysate Lane 2: 293T/17 whole cell lysate Lane 3: HepG2 whole cell lysate Lane 4: SH-SY5Y whole cell lysate Lane 5: human kidney lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 22 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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