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RAB7 Antibody (C-term)

Catalog # Availability Size / Price Inquiry
AMM07487G 100 μl / $495

RAB7 Antibody (C-term)

Brand

Leading Biology

Catalog Number

AMM07487G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality RAB7 antibody (C-term).

Molecular Weight

23490 Da

Cellular Localization

Antigen Cellular Localization: Late endosome. Lysosome. Cytoplasmic vesicle, phagosome. Melanosome. Cytoplasmic vesicle, phagosome membrane; Lipid- anchor; Cytoplasmic side. Note=Colocalizes with OSBPL1A at the late endosome. Found in the ruffled border (a late endosomal-like compartment in the plasma membrane) of bone-resorbing osteoclasts Recruited to phagosomes containing S.aureus or Mycobacterium (By similarity).

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

176-204 aa

Target

This RAB7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 176-204 amino acids from the C-terminal region of human RAB7.

Isotype

Rabbit Ig

Symbol

RAB7

GeneID

UniProt ID

Function

Key regulator in endo-lysosomal trafficking. Governs early-to-late endosomal maturation, microtubule minus-end as well as plus-end directed endosomal migration and positioning, and endosome-lysosome transport through different protein-protein interaction cascades. Plays a central role, not only in endosomal traffic, but also in many other cellular and physiological events, such as growth-factor-mediated cell signaling, nutrient- transportor mediated nutrient uptake, neurotrophin transport in the axons of neurons and lipid metabolism. Also involved in regulation of some specialized endosomal membrane trafficking, such as maturation of melanosomes, pathogen-induced phagosomes (or vacuoles) and autophagosomes. Plays a role in the maturation and acidification of phagosomes that engulf pathogens, such as S.aureus and M.tuberculosis. Plays a role in the fusion of phagosomes with lysosomes. Plays important roles in microbial pathogen infection and survival, as well as in participating in the life cycle of viruses. Microbial pathogens possess survival strategies governed by RAB7A, sometimes by employing RAB7A function (e.g. Salmonella) and sometimes by excluding RAB7A function (e.g. Mycobacterium). In concert with RAC1, plays a role in regulating the formation of RBs (ruffled borders) in osteoclasts. Controls the endosomal trafficking and neurite outgrowth signaling of NTRK1/TRKA. Regulates the endocytic trafficking of the EGF-EGFR complex by regulating its lysosomal degradation.

Summary

RAB family members are small, RAS-related GTP-binding proteins that are important regulators of vesicular transport. Each RAB protein targets multiple proteins that act in exocytic / endocytic pathways. RAB7 is a RAB family member that regulates vesicle traffic in the late endosomes and also from late endosomes to lysosomes. This protein is also involved in the cellular vacuolation of the VacA cytotoxin of Helicobacter pylori.

Form

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, IHC-P, FC, E

Dilution

WB~~1:2000 IHC-P~~1:50~100 FC~~1:10~50

Synonyms

Ras-related protein Rab-7a, RAB7A, RAB7

Images

All lanes : Anti-RAB7 Antibody (C-term) at 1:2000 dilution Lane 1: A431 whole cell lysate Lane 2: C2C12 whole cell lysate Lane 3: Hela whole cell lysate Lane 4: NIH/3T3 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 23 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes : Anti-RAB7 Antibody (C-term) at 1:2000 dilution Lane 1: NIH/3T3 whole cell lysate Lane 2: A431 whole cell lysate Lane 3: C2C12 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 23 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes : Anti-RAB7 Antibody (C-term) at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: NIH/3T3 whole cell lysate Lane 3: A431 whole cell lysate Lane 4: C2C12 whole cell lysate Lane 5: HepG2 whole cell lysate Lane 6: C6 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 23 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Specification

Quantity

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