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AOC3 Antibody (Center)

APR11425G Product Datasheet Protocol

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APR11425G		100 μl / $495

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Leading Biology Inc.

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AOC3 Antibody (Center)

Brand	Leading Biology	Catalog Number	APR11425G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality AOC3 antibody (Center).
Molecular Weight	84622 Da
Cellular Localization	Antigen Cellular Localization: Cell membrane; Single-pass type II membrane protein
Host	Rabbit
Species Reactivity	Human
Immunogen	613-640 aa
Target	This AOC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 613-640 amino acids from the Central region of human AOC3.
Isotype	Rabbit Ig
Symbol	VAP1
GeneID	8639
UniProt ID	Q16853
Function	Cell adhesion protein that participates in lymphocyte extravasation and recirculation by mediating the binding of lymphocytes to peripheral lymph node vascular endothelial cells in an L-selectin-independent fashion. Has semicarbazide-sensitive (SSAO) monoamine oxidase activity. May play a role in adipogenesis.
Summary	Copper amine oxidases catalyze the oxidative conversion of amines to aldehydes in the presence of copper and quinone cofactor. The product is a major protein on the adipocyte plasma membrane. It has adhesive properties and also has functional monoamine oxidase activity.
Form	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	IHC-P, WB, FC, E
Dilution	IHC-P~~1:10~50 WB~~1:1000 FC~~1:10~50
Synonyms	Membrane primary amine oxidase, Copper amine oxidase, HPAO, Semicarbazide-sensitive amine oxidase, SSAO, Vascular adhesion protein 1, VAP-1, AOC3, VAP1
Images	APR11425G staining AOC3 in human lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody. All lanes : Anti-AOC3 Antibody (Center) at 1:2000 dilution Lane 1: human lung lysate Lane 2: human liver lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 85 kDa Blocking/Dilution buffer: 5% NFDM/TBST. Western blot analysis of AOC3 Antibody (Center) (Cat. APR11425G) in CEM cell line lysates (35ug/lane).AOC3 (arrow) was detected using the purified Pab.
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