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CYP3A5 Antibody (C-term)

APG02895G Product Datasheet Protocol

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APG02895G		100 μl / $495

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Leading Biology Inc.

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CYP3A5 Antibody (C-term)

Brand	Leading Biology	Catalog Number	APG02895G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality CYP3A5 antibody (C-term).
Molecular Weight	57109 Da
Cellular Localization	Antigen Cellular Localization: Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein
Host	Rabbit
Species Reactivity	Human
Immunogen	476-502 aa
Target	This CYP3A5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 476-502 amino acids from the C-terminal region of human CYP3A5.
Isotype	Rabbit Ig
GeneID	1577
UniProt ID	P20815
Function	Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
Summary	CYP3A5 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by glucocorticoids and some pharmacological agents. The enzyme metabolizes drugs such as nifedipine and cyclosporine as well as the steroid hormones testosterone, progesterone and androstenedione.
Form	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, IHC-P, IF, E
Dilution	WB~~1:1000 IHC-P~~1:10~50 IF~~1:10~50
Synonyms	Cytochrome P450 3A5, CYPIIIA5, Cytochrome P450 HLp2, Cytochrome P450-PCN3, CYP3A5
Images	Western blot analysis of lysate from SK-BR-3 cell line, using CYP3A5 Antibody (C-term) ( APG02895G). APG02895G was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug. Western blot analysis of anti-CYP3A5 Antibody (C-term) (Cat. APG02895G) in 293 cell line lysates (35ug/lane). CYP3A5 (arrow) was detected using the purified Pab. Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with CYP3A5 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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