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HADHA Antibody (C-term)

AMM05282G Product Datasheet Protocol

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AMM05282G		100 μl / $495

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Leading Biology Inc.

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HADHA Antibody (C-term)

Brand	Leading Biology	Catalog Number	AMM05282G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality HADHA antibody (C-term).
Molecular Weight	83000 Da
Cellular Localization	Antigen Cellular Localization: Mitochondrion.
Host	Rabbit
Species Reactivity	Human
Immunogen	737-763 aa
Target	This HADHA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 737-763 amino acids from the C-terminal region of human HADHA.
Isotype	Rabbit Ig
Symbol	HADH
GeneID	3030
UniProt ID	P40939
Function	Bifunctional subunit.
Summary	HADHA is the alpha subunit of the mitochondrial trifunctional protein, which catalyzes the last three steps of mitochondrial beta-oxidation of long chain fatty acids. The mitochondrial membrane-bound heterocomplex is composed of four alpha and four beta subunits, with the alpha subunit catalyzing the 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities.
Form	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, IHC-P, FC, E
Dilution	WB~~1:1000 IHC-P~~1:10~50 FC~~1:10~50
Synonyms	Trifunctional enzyme subunit alpha, mitochondrial, 78 kDa gastrin-binding protein, TP-alpha, Long-chain enoyl-CoA hydratase, Long chain 3-hydroxyacyl-CoA dehydrogenase, HADHA, HADH
Images	All lanes : Anti-HADHA Antibody (C-term) at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: Jurkat whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 83 kDa Blocking/Dilution buffer: 5% NFDM/TBST. HADHA Antibody (C-term) (Cat. AMM05282G) western blot analysis in A431 cell line lysates (35ug/lane).This demonstrates the HADHA antibody detected the HADHA protein (arrow). Formalin-fixed and paraffin-embedded human lung carcinoma reacted with HADHA Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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