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ENO1 Antibody (Center)

Catalog # Availability Size / Price Inquiry
APR07696G 100 μl / $495

ENO1 Antibody (Center)

Brand

Leading Biology

Catalog Number

APR07696G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality ENO1 antibody (Center).

Molecular Weight

47169 Da

Cellular Localization

Antigen Cellular Localization: Cytoplasm. Cell membrane. Cytoplasm, myofibril, sarcomere, M line. Note=Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. ENO1 is localized to the M line

Host

Rabbit

Species Reactivity

Human, Mouse

Immunogen

178-205 aa

Target

This ENO1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 178-205 amino acids from the Central region of human ENO1.

Isotype

Rabbit Ig

Symbol

ENO1L1, MBPB1, MPB1

GeneID

UniProt ID

Function

Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.

Summary

ENO1 is one of three enolase isoenzymes found in mammals; it is alpha-enolase, a homodimeric soluble enzyme, and also a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length protein, the isoenzyme, is found in the cytoplasm. The shorter protein is produced from an alternative translation start, is localized to the nucleus, and has been found to bind to an element in the c-myc promoter.

Form

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, IHC-P, FC, IF, E

Dilution

WB~~1:1000 IHC-P~~1:10~50 FC~~1:10~50 IF~~1:10~50

Synonyms

Alpha-enolase, 2-phospho-D-glycerate hydro-lyase, C-myc promoter-binding protein, Enolase 1, MBP-1, MPB-1, Non-neural enolase, NNE, Phosphopyruvate hydratase, Plasminogen-binding protein, ENO1, ENO1L1, MBPB1, MPB1

Images

ENO1 Antibody (Center) (Cat. APR07696G) western blot analysis in Jurkat,MCF-7,U251,mouse NIH/3T3 cell line and mouse brain tissue lysates (35ug/lane).This demonstrates the ENO1 antibody detected the ENO1 protein (arrow).

Formalin-fixed and paraffin-embedded human lymph reacted with ENO1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

ENO1 Antibody (Center) (Cat. APR07696G) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

Specification

Quantity

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