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EDA Antibody (N-term)

AMM06999G Product Datasheet Protocol

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AMM06999G		100 μl / $495

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Leading Biology Inc.

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EDA Antibody (N-term)

Brand	Leading Biology	Catalog Number	AMM06999G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality EDA antibody (N-term).
Molecular Weight	41294 Da
Cellular Localization	Antigen Cellular Localization: Cell membrane; Single-pass type II membrane protein
Host	Rabbit
Species Reactivity	Human
Immunogen	47-76 aa
Target	This EDA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 47-76 amino acids from the N-terminal region of human EDA.
Isotype	Rabbit Ig
Symbol	ED1, EDA2
GeneID	1896
UniProt ID	Q92838
Function	Seems to be involved in epithelial-mesenchymal signaling during morphogenesis of ectodermal organs. Isoform 1 binds only to the receptor EDAR, while isoform 3 binds exclusively to the receptor XEDAR.
Summary	EDA is a type II membrane protein that can be cleaved by furin to produce a secreted form. This protein, which belongs to the tumor necrosis factor family, acts as a homotrimer and may be involved in cell-cell signaling during the development of ectodermal organs. Defects in the gene for EDA are a cause of ectodermal dysplasia, anhidrotic, which is also known as X-linked hypohidrotic ectodermal dysplasia.
Form	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, IHC-P, FC, E
Dilution	WB~~1:1000 IHC-P~~1:10~50 FC~~1:10~50
Synonyms	Ectodysplasin-A, Ectodermal dysplasia protein, EDA protein, Ectodysplasin-A, membrane form, Ectodysplasin-A, secreted form, EDA, ED1, EDA2
Images	Western blot analysis of lysate from RD cell line,using EDA Antibody (N-term)(Cat. AMM06999G).AMM06999G was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysate at 35ug per lane. Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with EDA antibody (N-term) (Cat. AMM06999G), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Flow cytometric analysis of hela cells using EDA Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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