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SFN Antibody (C-term)

APR13300G Product Datasheet Protocol

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APR13300G		100 μl / $495

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Leading Biology Inc.

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SFN Antibody (C-term)

Brand	Leading Biology	Catalog Number	APR13300G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality SFN antibody (C-term).
Molecular Weight	27774 Da
Cellular Localization	Antigen Cellular Localization: Cytoplasm. Nucleus. Secreted. Note=May be secreted by a non-classical secretory pathway
Host	Rabbit
Species Reactivity	Human
Immunogen	222-248 aa
Target	This SFN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 222-248 amino acids from the C-terminal region of human SFN.
Isotype	Rabbit Ig
Symbol	HME1
GeneID	2810
UniProt ID	P31947
Function	Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53.
Form	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, IHC-P, IF, FC, E
Dilution	WB~~1:1000 IHC-P~~1:10~50 IF~~1:10~50 FC~~1:10~50
Synonyms	14-3-3 protein sigma, Epithelial cell marker protein 1, Stratifin, SFN, HME1
Images	Western blot analysis of lysates from A431, Hela cell line (from left to right), using SFN Antibody (C-term) ( APR13300G). APR13300G was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane. SFN antibody (C-term) (Cat. APR13300G) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SFN antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated. Confocal immunofluorescent analysis of SFN Antibody (C-term)(Cat. APR13300G) with A549 cell followed by Alexa Fluor搴?488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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