> Antigen, Antibodies, ELISA, Western Blot > Primary Antibody > Polyclonal Antibodies > AKR1B1 Antibody (Center)Brand |
Leading Biology | Catalog Number |
APG01653G |
Product Type |
Polyclonal Antibodies | Field of Research |
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Product Overview |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality AKR1B1 antibody (Center).
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Molecular Weight |
35853 Da
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Cellular Localization |
Antigen Cellular Localization:
Cytoplasm.
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Host |
Rabbit
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Species Reactivity |
Human
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Immunogen |
102-135 aa
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Target |
This AKR1B1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 102-135 amino acids from the Central region of human AKR1B1.
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Isotype |
Rabbit Ig
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Symbol |
ALDR1
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GeneID |
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UniProt ID |
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Function |
Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols with a broad range of catalytic efficiencies.
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Summary |
AKR1B1 is a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This protein catalyzes the reduction of a number of aldehydes, including the aldehyde form of glucose, and is thereby implicated in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol.
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Form |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
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Storage & Stability |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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Applications |
WB, IF, IHC-P, E
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Dilution |
WB~~1:1000
IF~~1:10~50
IHC-P~~1:10~50
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Synonyms |
Aldose reductase, AR, Aldehyde reductase, Aldo-keto reductase family 1 member B1, AKR1B1, ALDR1
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Images |
All lanes : Anti-AKR1B1 Antibody (Center) at 1:8000 dilution Lane 1: A431 whole cell lysate Lane 2: A549 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 36 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot analysis of anti-AKR1B1 Pab (Cat. APG01653G) in Jurkat cell line lysates (35ug/lane).AKR1B1 (arrow) was detected using the purified Pab.
Western blot analysis of AKR1B1 (arrow) using rabbit polyclonal AKR1B1 Antibody (Center) (Cat. APG01653G). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the AKR1B1 gene. |
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Specification |
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Quantity |
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Leading Biology Inc.
2600 Hilltop DR, Building G, B Suite C138
Richmond, CA, 94806
Tel: 1-661-524(LBI)-0262
Email: info@leadingbiology.com
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