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Aldolase (ALDOA) Antibody (N-term)

APG01809G Product Datasheet Protocol

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APG01809G		100 μl / $495

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Leading Biology Inc.

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Aldolase (ALDOA) Antibody (N-term)

Brand	Leading Biology	Catalog Number	APG01809G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality Aldolase (ALDOA) antibody (N-term).
Molecular Weight	39420 Da
Cellular Localization	Antigen Cellular Localization: Cytoplasm, myofibril, sarcomere, I band. Cytoplasm, myofibril, sarcomere, M line. Note=In skeletal muscle, accumulates around the M line and within the I band, colocalizing with FBP2 on both sides of the Z line in the absence of Ca(2+)
Host	Rabbit
Species Reactivity	Human
Immunogen	66-95 aa
Target	This Aldolase (ALDOA) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 66-95 amino acids from the N-terminal region of human Aldolase (ALDOA).
Isotype	Rabbit Ig
Symbol	ALDA
GeneID	226
UniProt ID	P04075
Function	Plays a key role in glycolysis and gluconeogenesis. In addition, may also function as scaffolding protein (By similarity).
Summary	Aldolase A (fructose-bisphosphate aldolase) is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Aldolase A is found in the developing embryo and is produced in even greater amounts in adult muscle. Aldolase A expression is repressed in adult liver, kidney and intestine and similar to aldolase C levels in brain and other nervous tissue. Aldolase A deficiency has been associated with myopathy and hemolytic anemia.
Form	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	IF, IHC-P, WB, E
Dilution	WB~~1:1000 IHC-P~~1:10~50 IF~~1:10~50
Synonyms	Fructose-bisphosphate aldolase A, Lung cancer antigen NY-LU-1, Muscle-type aldolase, ALDOA, ALDA
Images	Western blot analysis of lysates from 293,RD,mouse NIH/3T3,rat L6 cell line (from left to right),using ALDOA Antibody (N-term)(Cat. APG01809G).APG01809G was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane. Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with ALDOA antibody (N-term) (Cat. APG01809G), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Confocal immunofluorescent analysis of Aldolase (ALDOA) Antibody (N-term)( APG01809G) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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