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GNG2 Antibody (N-Term)

AMRa00031G Product Datasheet Protocol

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AMRa00031G		100 μl / $495

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Leading Biology Inc.

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GNG2 Antibody (N-Term)

Brand	Leading Biology	Catalog Number	AMRa00031G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality GNG2 antibody (N-Term).
Molecular Weight	7850 Da
Cellular Localization	Antigen Cellular Localization: Cell membrane; Lipid-anchor; Cytoplasmic side
Host	Rabbit
Species Reactivity	Human, Mouse
Target	This GNG2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 19-52 amino acids from of human GNG2.
Isotype	Rabbit Ig
GeneID	54331
UniProt ID	P59768
Function	Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein- effector interaction (By similarity).
Summary	Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein- effector interaction (By similarity).
Form	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	IHC-P, WB, E
Dilution	IHC-P~~1:25 WB~~1:8000
Synonyms	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2, G gamma-I, GNG2
Images	AMRa00031G staining GNG2 in human brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody. All lanes : Anti-GNG2 Antibody (N-Term) at 1:8000 dilution Lane 1: human brain lysate Lane 2: mouse brain lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 8 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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