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Antigen, Antibodies, ELISA, Western Blot >
Primary Antibody >
Polyclonal Antibodies >
PYGL Antibody (C-Term)Brand |
Leading Biology | Catalog Number |
AMR09672G |
Product Type |
Polyclonal Antibodies | Field of Research |
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Product Overview |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality PYGL antibody (C-Term).
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Molecular Weight |
97149 Da
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Host |
Rabbit
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Species Reactivity |
Human, Mouse
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Target |
This PYGL antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 809-843 amino acids from human PYGL.
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Isotype |
Rabbit Ig
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GeneID |
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UniProt ID |
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Function |
Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties.
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Summary |
Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties.
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Form |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
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Storage & Stability |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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Applications |
IHC-P, WB, E
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Dilution |
IHC-P~~1:25
WB~~1:2000
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Synonyms |
Glycogen phosphorylase, liver form, PYGL
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Images |
AMR09672G staining PYGL in human liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody. All lanes : Anti-PYGL Antibody (C-Term) at 1:2000 dilution Lane 1: human liver lysate Lane 2: Hela whole cell lysate Lane 3: HepG2 whole cell lysate Lane 4: NIH/3T3 whole cell lysate Lane 5: PC-3 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 97 kDa Blocking/Dilution buffer: 5% NFDM/TBST. |
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Specification |
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Quantity |
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