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BCL9 Antibody (N-Term)

Catalog # Availability Size / Price Inquiry
AMM08755G 100 μl / $495

BCL9 Antibody (N-Term)

Brand

Leading Biology

Catalog Number

AMM08755G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality BCL9 antibody (N-Term).

Molecular Weight

149290 Da

Cellular Localization

Antigen Cellular Localization: Nucleus.

Host

Rabbit

Species Reactivity

Human, Mouse

Target

This BCL9 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 272-304 amino acids from human BCL9.

Isotype

Rabbit Ig

GeneID

UniProt ID

Function

Involved in signal transduction through the Wnt pathway. Promotes beta-catenin's transcriptional activity (By similarity).

Summary

Involved in signal transduction through the Wnt pathway. Promotes beta-catenin's transcriptional activity (By similarity).

Form

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

IF, WB, E

Dilution

IF~~1:25 WB~~1:2000

Synonyms

B-cell CLL/lymphoma 9 protein, B-cell lymphoma 9 protein, Bcl-9, Protein legless homolog, BCL9

Images

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling Pdx1 with AMM08755G at 1/25 dilution, followed by Dylight? 488-conjugated goat anti-rabbit IgG (NK179883) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing nucleus staining on HeLa cell line. Cytoplasmic actin is detected with Dylight? 554 Phalloidin (PD18466410) at 1/100 dilution (red).

All lanes : Anti-BCL9 Antibody (N-Term) at 1:2000 dilution Lane 1: mouse spleen lysates Lane 2: human testis lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 149 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Specification

Quantity

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