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PGM1 Antibody (C-Term)

APR09092G Product Datasheet Protocol

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APR09092G		100 μl / $495

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Leading Biology Inc.

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PGM1 Antibody (C-Term)

Brand	Leading Biology	Catalog Number	APR09092G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality PGM1 antibody (C-Term).
Molecular Weight	61449 Da
Cellular Localization	Antigen Cellular Localization: Isoform 1: Cytoplasm.
Host	Rabbit
Species Reactivity	Human, Mouse
Target	This PGM1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 469-501 amino acids from human PGM1.
Isotype	Rabbit Ig
GeneID	5236
UniProt ID	P36871
Function	This enzyme participates in both the breakdown and synthesis of glucose.
Summary	This enzyme participates in both the breakdown and synthesis of glucose.
Form	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, IHC-P, E
Dilution	WB~~1:2000 IHC-P~~1:25
Synonyms	Phosphoglucomutase-1, PGM 1, Glucose phosphomutase 1, PGM1
Images	All lanes : Anti-PGM1 Antibody (C-Term) at 1:2000 dilution Lane 1: human liver lysate Lane 2: mouse heart lysate Lane 3: Jurkat whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 61 kDa Blocking/Dilution buffer: 5% NFDM/TBST. APR09092G staining PGM1 in human liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody. APR09092G staining PGM1 in human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
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