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UCHL3 Antibody (C-term)

APR11260G Product Datasheet Protocol

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APR11260G		100 μl / $495

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Leading Biology Inc.

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UCHL3 Antibody (C-term)

Brand	Leading Biology	Catalog Number	APR11260G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality UCHL3 antibody (C-term).
Molecular Weight	26183 Da
Cellular Localization	Antigen Cellular Localization: Cytoplasm.
Host	Rabbit
Species Reactivity	Human
Immunogen	195-225 aa
Target	This UCHL3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 195-225 amino acids from the C-terminal region of human UCHL3.
Isotype	Rabbit Ig
GeneID	7347
UniProt ID	P15374
Function	Deubiquitinating enzyme (DUB) that controls levels of cellular ubiquitin through processing of ubiquitin precursors and ubiquitinated proteins. Thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. Has a 10-fold preference for Arg and Lys at position P3", and exhibits a preference towards 'Lys-48'-linked Ubiquitin chains. Deubiquitinates ENAC in apical compartments, thereby regulating apical membrane recycling. Indirectly increases the phosphorylation of IGFIR, AKT and FOXO1 and promotes insulin- signaling and insulin-induced adipogenesis. Required for stress- response retinal, skeletal muscle and germ cell maintenance. May be involved in working memory. Can hydrolyze UBB(+1), a mutated form of ubiquitin which is not effectively degraded by the proteasome and is associated with neurogenerative disorders.
Summary	Covalent attachment of the C-terminus of ubiquitin to cellular proteins plays a role in a variety of cellular processes. Ubiquitin C-terminal hydrolysis is catalyzed by deubiquitinating (DUB) enzymes and is necessary for several functions, including liberation of monomeric ubiquitin from the precursors encoded by ubiquitin genes and recycling of ubiquitin monomers. There are 2 distinct families of DUBs, ubiquitin-specific proteases (UBPs) and ubiquitin C-terminal hydrolases (UCHs). Mayer and Wilkinson (1989) identified 4 distinct UCH activities from bovine thymus. All 4 were thiol proteases and had high-affinity binding sites for ubiquitin. Wilkinson et al. (1989) purified the predominant isozyme, UCHL3, and raised antibodies against it. By screening a human B-cell expression library with the antibodies, the authors isolated cDNAs encoding human UCHL3. Sequence comparisons revealed that the sequence of the predicted 230-amino acid human UCHL3 protein is 54% identical to that of UCHL1.
Form	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, IHC-P, E
Dilution	WB~~1:1000 IHC-P~~1:10~50
Synonyms	Ubiquitin carboxyl-terminal hydrolase isozyme L3, UCH-L3, Ubiquitin thioesterase L3, UCHL3
Images	Western blot analysis of lysates from 293, 293T, SW620, U-87 MG cell line (from left to right), using UCHL3 Antibody (C209) ( AP2127B). AP2127B was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane. Western blot analysis of UCHL3 (Cat. APR11260G) in HepG2 cell line lysates (35ug/lane). UCHL3 (arrow) was detected using the purified Pab. Western blot analysis of his tag antibody in UCHL3 gene transfected 293 lysates (35ug/lane). His-tag UCHL3 protein (arrow) is detected.
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