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NOVA1 Antibody (Center)

AMM06761G Product Datasheet Protocol

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AMM06761G		100 μl / $495

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Leading Biology Inc.

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NOVA1 Antibody (Center)

Brand	Leading Biology	Catalog Number	AMM06761G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality NOVA1 antibody (Center).
Molecular Weight	52056 Da
Cellular Localization	Antigen Cellular Localization: Nucleus.
Host	Rabbit
Species Reactivity	Human
Target	This NOVA1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 139-173 amino acids from the Central region of human NOVA1.
Isotype	Rabbit Ig
GeneID	4857
UniProt ID	P51513
Function	May regulate RNA splicing or metabolism in a specific subset of developing neurons.
Summary	May regulate RNA splicing or metabolism in a specific subset of developing neurons.
Form	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, IHC-P, E
Dilution	IHC-P~~1:25 WB~~1:1000
Synonyms	RNA-binding protein Nova-1, Neuro-oncological ventral antigen 1, Onconeural ventral antigen 1, Paraneoplastic Ri antigen, Ventral neuron-specific protein 1, NOVA1
Images	Immunohistochemical analysis of paraffin-embedded H. astroglioma section using NOVA1 Antibody (Center)( AMM06761G). AMM06761G was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining. All lanes : Anti-NOVA1 Antibody (Center)(AMM06761G) at 1:1000 dilution Lane 1: A431 whole cell lysates Lane 2: Hela whole cell lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 49 kDa Blocking/Dilution buffer: 5% NFDM/TBST. Western blot analysis of lysates from A431, Hela cell line (from left to right), using NOVA1 Antibody (Center)( AMM06761G). AMM06761G was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
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