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ATP5I Antibody (C-term)

APG02134G Product Datasheet Protocol

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APG02134G		100 μl / $495

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Leading Biology Inc.

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ATP5I Antibody (C-term)

Brand	Leading Biology	Catalog Number	APG02134G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality ATP5I antibody (C-term).
Molecular Weight	7933 Da
Cellular Localization	Antigen Cellular Localization: Mitochondrion. Mitochondrion inner membrane.
Host	Rabbit
Species Reactivity	Human, Mouse
Target	This ATP5I antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 55-89 amino acids from the C-terminal region of human ATP5I.
Isotype	Rabbit Ig
Symbol	ATP5K
GeneID	521
UniProt ID	P56385
Function	Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain. Minor subunit located with subunit a in the membrane.
Summary	Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain. Minor subunit located with subunit a in the membrane.
Form	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, IHC-P, IF, E
Dilution	IF~~1:25 IHC-P~~1:25 WB~~1:1000
Synonyms	ATP synthase subunit e, mitochondrial, ATPase subunit e, ATP5I, ATP5K
Images	Fluorescent image of Hela cells stained with ATP5I Antibody (C-term)( APG02134G). APG02134G was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue). Cytoplasmic actin was counterstained with Alexa Fluor? 555 conjugated with Phalloidin (red). Immunohistochemical analysis of paraffin-embedded H. liver section using ATP5I Antibody (C-term)( APG02134G). APG02134G was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining. Fluorescent image of Hela cells stained with ATP5I Antibody (C-term)( APG02134G). APG02134G was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue). Cytoplasmic actin was counterstained with Alexa Fluor? 555 conjugated with Phalloidin (red).
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