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CFL2 Antibody (C-term)

APR15415G Product Datasheet Protocol

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APR15415G		100 μl / $495

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Leading Biology Inc.

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CFL2 Antibody (C-term)

Brand	Leading Biology	Catalog Number	APR15415G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality CFL2 antibody (C-term).
Molecular Weight	18737 Da
Cellular Localization	Antigen Cellular Localization: Nucleus matrix. Cytoplasm, cytoskeleton
Host	Rabbit
Species Reactivity	Human, Mouse
Target	This CFL2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 130-144 amino acids from the C-terminal region of human CFL2.
Isotype	Rabbit Ig
GeneID	1073
UniProt ID	Q9Y281
Function	Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods (By similarity).
Summary	Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods (By similarity).
Form	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, IF, E
Dilution	IF~~1:25 WB~~1:1000
Synonyms	Cofilin-2, Cofilin, muscle isoform, CFL2
Images	Fluorescent image of Hela cells stained with CFL2 Antibody (C-term)( APR15415G). APR15415G was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor? 555 conjugated with Phalloidin (red). Western blot analysis of lysates from human skeletal muscle and mouse brain tissue lysate(from left to right), using CFL2 Antibody (C-term)( APR15415G). APR15415G was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
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